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Disinfection method of mariculture water body

A technology for mariculture and disinfection methods, applied in chemical instruments and methods, animal husbandry wastewater treatment, light water/sewage treatment, etc., can solve problems such as complex components and lack of research

Active Publication Date: 2022-05-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems that need to be further explored and solved in this technology, such as the control of operating conditions in the disinfection process, the inactivation mechanism of microorganisms, efficient and economical SO 4 - Selection of activation method, etc.
Also, on SO 4 - There are no reports on the application research in water disinfection before stocking seedlings in recirculating mariculture and the inactivation mechanism of various pathogenic microorganisms in the water.
Moreover, the composition of mariculture water is much more complex than that of fresh water, and there are a large number of various anions in it. The influence of different physical and chemical conditions on various pathogenic microorganisms, and the influence of the same pathogenic microorganism by different physical and chemical conditions are also lacking.

Method used

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  • Disinfection method of mariculture water body
  • Disinfection method of mariculture water body
  • Disinfection method of mariculture water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The frozen Streptococcus agalactiae species stored in the -80°C refrigerator were thawed and then inoculated in BHI solid nutrient broth medium, and activated at 37°C. After a single colony grows well, pick it with an inoculation loop and inoculate it in 5 mL of BHI liquid nutrient broth medium, shake it at 37 °C and the rotation speed is 300 r / min until it reaches the logarithmic growth stage, and take out 1 mL of bacterial liquid to Centrifuge at 4000 r / min for 5 minutes, then resuspend the precipitate in sterilized saline, repeat the operation twice, dissolve the precipitated bacterial mass in 100 mL of sterilized aquaculture water to prepare 10 7 CFU / mL suspension.

Embodiment 2

[0026] Formulated with Cl - , HCO 3 - , SO 4 2- , Br - The concentrations of 10.489g / L, 65.5mg / L, 1.35g / L, and 27.9mg / L were used to simulate real aquaculture water, and the pH value of the aquaculture water was not adjusted. Prepare 0.05mmol / L, 0.10mmol / L, 0.15mmol / L, 0.20mmol / L, 0.25mmol / L, 0.30mmol / L sodium persulfate solution respectively, and fix the UV intensity to 30uw / cm 2 , after turning on the 10W low-pressure mercury lamp for a period of time, the prepared 10 7 Pipette 30 mL of CFU / mL S. agalactiae suspension into a translucent quartz reaction tube in a sterile state, put a magnetite, add 300 uL of sodium persulfate solutions of different concentrations, and put the quartz reaction tube into the In the UV-stabilized reactor, take out the reaction tube for sampling at different times of reaction, dilute the sample in a sterile ultra-clean workbench, streak the corresponding solid medium, and put it in a 37°C incubator for upside-down culture. After 24 hours, t...

Embodiment 3

[0029] Prepare artificial aquaculture water in the same manner as in Example 2, prepare a sodium persulfate solution with a concentration of 0.1 mmol / L, adjust the artificial aquaculture water with pH values ​​of 5, 6, 7, 8, and 9 respectively, and fix it at 30uw / ultraviolet intensity. cm 2 , after turning on the 10W low-pressure mercury lamp to stabilize for a period of time, the remaining reaction steps are the same as in Example 2, and the inactivation rate of UV / PS to Streptococcus agalactiae in artificial mariculture water with different pH values ​​is calculated.

[0030] like figure 2 As shown, the experiment found that when the reaction time was the same, the inactivation efficiency of Streptococcus agalactiae was the highest when the pH was 5, while the inactivation efficiency was the lowest when the pH was 9. With the change of pH from 9 to 5, UV The inactivation effect of / PS on Streptococcus agalactiae was gradually enhanced. SO in the system under acidic and ne...

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Abstract

The invention discloses a novel disinfection method for mariculture water, which uses a UV / PS advanced oxidation method to carry out disinfection and inactivation treatment on the mariculture water containing Streptococcus agalactiae. The novel disinfection method for mariculture water of the present invention can effectively inactivate Gram-positive bacteria-Streptococcus agalactiae (S. agalactiae) in the mariculture water, and no toxic and harmful substances and secondary pollution will be produced during the disinfection process. To sum up, the present invention is a highly efficient and reliable mariculture water disinfection method with unique advantages, simple preparation technology, low cost, extremely fast kill of microorganisms.

Description

technical field [0001] The invention belongs to the technical field of water pollution disinfection, and in particular relates to a novel method for disinfection of aquaculture water bodies. Background technique [0002] Marine aquaculture is a traditional advantageous industry in my country's coastal areas, which not only drives the economic and social development of fishing areas, but also meets the needs of urban and rural residents' diversified dietary nutrition structure. According to statistics, the total output of marine aquaculture in China in 2017 reached 20.007 million tons. The circulating mariculture system treats and recycles the used water after the products are salvaged every season, reducing the consumption of seawater and freshwater resources, and has been widely used. In the recirculating seawater aquaculture pond, seawater will bring in various pathogenic bacteria; on the other hand, after stocking fish and shrimp, after a period of aquaculture, the accum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/50C02F1/32C02F103/20
CPCC02F1/50C02F1/32C02F2103/20C02F2303/04
Inventor 张媛媛韦敏黄坤玲朱云杰余克服
Owner GUANGXI UNIV
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