Geotrichum candidum and bean dreg fermented product and preparation method thereof
A technology for fermented products and Geotrichum candidum, which is applied in the field of Geotrichum candidum, bean dregs fermented products and their preparation, can solve the problem of low fat degradation rate, and achieve the effects of reducing the content of fat and excellent degradability.
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Embodiment 1
[0038] Preparation of seed solution: Store Geotrichum candidum CGMCC No.21454 at -80°C and spread it on potato dextrose agar (PDA) medium, and cultivate at 28°C for 36h for activation; take the activated mycelium and inoculate it into 100mL potato dextrose broth (PDB). ) medium, 28℃, 210r·min -1 Shaker culture for 2-3d, secondary activation; 5500r·min -1 The cells were collected by centrifugation for 15 min, and the volume was adjusted to 1 g·mL with sterile water. -1 Use as seed solution.
[0039] Preparation of fermentation medium: take 5.5g of bean dregs, add 44mL of water (solid-to-liquid ratio 1:8) to homogenize, add 2g of starch and mix, adjust pH to 4.5, dilute to 50mL, and transfer it to a 250mL conical flask (filling liquid). 20%), sterilized at 121 °C, 0.1 MPa for 15 min, and cooled for later use.
[0040]Fermentation of bean dregs: The seed liquid of Geotrichum candidum CGMCC No.21454 was placed in the fermentation medium at an inoculation amount of 1 g / mL, at 30...
Embodiment 2
[0042] Take 100 grams of dry bean dregs, cool to 38-42 ° C after sterilization, add the seed solution of Geotrichum candidum CGMCC No. 21454 prepared in Example 1, stir evenly, put into a plastic bag, poke holes, and ferment at 30 ° C to prepare bean dregs Fermented products. Wherein, the concentration of Geotrichum candidum in the seed solution is 1g / mL, and the addition amount of the seed solution is 10mL relative to 1kg of bean dregs,
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