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Bacterial strain, extract, nano-pesticide and application and preparation method of nano-pesticide

A technology of nano-pesticides and extracts, applied in the fields of extracts, nano-pesticides and their application and preparation, and bacterial strains, can solve the problems of high environmental microbial toxicity and environmental pollution, achieve high antibacterial activity and high efficiency, low cost, Effect of low organic solvent content

Active Publication Date: 2021-06-11
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above deficiencies in the prior art, the object of the present invention is to provide bacterial strains, extracts, nano-pesticides and their application and preparation methods, aiming to solve the problems that existing chemical pesticides are easy to cause environmental pollution and have high toxicity to environmental microorganisms

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  • Bacterial strain, extract, nano-pesticide and application and preparation method of nano-pesticide
  • Bacterial strain, extract, nano-pesticide and application and preparation method of nano-pesticide
  • Bacterial strain, extract, nano-pesticide and application and preparation method of nano-pesticide

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preparation example Construction

[0048] Based on this, the embodiment of the present invention also provides a preparation method of nano-pesticide, which includes the steps of:

[0049] S10, activating the bacterial strain R64 to obtain a single colony of activated R64;

[0050] S20. Fermenting and extracting the activated R64 single colony in sequence to obtain an extract of bacterial strain R64;

[0051] S30, combining the extract of the bacterial strain R64 with AgNO 3 The solutions are mixed and reacted to obtain the nanopesticide.

[0052] This example provides a bacterial strain R64 that can produce natural antibacterial products, and obtains the extract of bacterial strain R64 with antibacterial activity through activation, fermentation and extraction, and uses the extract of bacterial strain R64 as a raw material, by preparing into nano The pesticide method effectively reduces the environmental toxicity of the extract of strain R64, and at the same time increases its antibacterial activity and high...

Embodiment 1

[0080] Embodiment 1, the activation of bacterium, fungus

[0081] 1. Isolation, purification and identification of strain R64:

[0082] Obtain samples from the surface soil (5cm depth) of Hong Kong Mipu mangrove forest, soak a part with sterile water, and take the supernatant (leaching solution) after a period of time;

[0083] The soil leachate was diluted in multiples of 10, 100, 1000, and 10,000, spread on the MA medium with a spreader, and cultivated in a 28°C incubator for 48 hours;

[0084] Pick a single colony and inoculate it on a new MA plate by streaking (if there is no single colony on the coated plate, continue to dilute with a 10-fold gradient until a single colony appears), and incubate in a 28°C incubator for 48 hours ;

[0085] After PCR (two-way primers, 27F: AGAGTTTGATCMTGGCTCAG; 1492R: GGTTACCTTGTTACGACTT), it was sequenced by Qingke Biological Company.

[0086] After the identification results were compared by NCBI, it was found that the genus of R64 was...

Embodiment 2

[0094] The preparation of the extract of embodiment 2, bacterial strain R64

[0095] 1. Fermentation of strain R64:

[0096] Add 200 mL of SGTYP medium to a 500 mL Erlenmeyer flask, inoculate the activated R64 single colony, and culture at 28°C and 200 rpm for 24 hours, then transfer 50 mL to a 2L Erlenmeyer flask containing 800 mL of SGTYP, and culture at room temperature for 4 days at 200 rpm . SGTYP medium is composed of soluble starch 5.0g, glucose 5.0g, microbial peptone 1.0g, tryptone 1.0g, yeast extract 1g, artificial sea salt 17g, distilled water 1L (prepared in the laboratory, pH range is 7.6±0.2. Starch and glucose The production company of Aladdin; the production company of microbial peptone, tryptone and yeast extract is OXOID; the production company of artificial sea salt is Yier.)

[0097] 2. Extraction of the extract of bacterial strain R64:

[0098] Add 3 times the volume of ethyl acetate to the fermented bacterial strain R64 fermentation broth, shake and mix ...

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Abstract

The invention discloses a bacterial strain, an extract, a nano-pesticide and application and a preparation method of the nano-pesticide. The bacterial strain is a bacterial strain R64 from mangrove forest, the bacterial strain R64 is classified and named as Pseudomonasaeruginosa, and the preservation number of the bacterial strain R64 is CGMCC No. 21803. The extract of the bacterial strain R64 extracted from the bacterial strain R64 and the nano pesticide synthesized by using the extract of the bacterial strain R64 have high antibacterial activity and high efficiency, and have low toxicity to environmental microorganisms. Meanwhile, the biological preparation method disclosed by the invention is low in cost, simple to operate and relatively environment-friendly. The invention provides a novel natural product pesticide, and meanwhile, the environmental toxicity of the natural product pesticide is reduced and the antibacterial activity and high efficiency of the natural product pesticide are improved through a method for preparing the nano pesticide.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to bacterial strains, extracts, nanopesticides and their application and preparation methods. Background technique [0002] Plant diseases caused by pathogenic microorganisms, especially fungal diseases on staple food crops, seriously threaten agricultural production and have always been a limiting factor restricting the safety and yield of agricultural products. However, most crops currently lack varieties with good resistance to plant pathogenic bacteria, and the control of plant diseases still relies on traditional chemical control methods, including the use of agricultural antibiotics, inorganic copper, organic copper and thiazole preparations. However, the use of traditional chemical pesticides not only easily leads to problems such as environmental pollution, pesticide residues, and high toxicity to environmental microorganisms, but also promotes the emergence of drug-resistant st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P1/00A01P3/00C12R1/385
CPCA01N63/27
Inventor 郝凌云徐颖黄刚尚琛晶
Owner SHENZHEN UNIV
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