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Bioactive glass/bionic functional polypeptide complex as well as preparation method and application thereof

A technology of bioactive glass and bionic functions, which can be used in drug combination, liquid delivery, and pharmaceutical formulations. It can solve the problems of lack of remineralization calcium and phosphorus sources, inability to induce new generation of dental pulp cells, etc., to promote dentin repair, promote Proliferation Migration and Differentiation, Effects of Design Science

Active Publication Date: 2021-06-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One of the purposes of the present invention is to provide a biologically active glass / bionic functional polypeptide complex to solve the problem that the biomimetic mineralization functional polypeptide gel preparations in the prior art lack the necessary calcium and phosphorus sources for remineralization and cannot induce dental pulp cells nascent technical issues

Method used

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  • Bioactive glass/bionic functional polypeptide complex as well as preparation method and application thereof
  • Bioactive glass/bionic functional polypeptide complex as well as preparation method and application thereof
  • Bioactive glass/bionic functional polypeptide complex as well as preparation method and application thereof

Examples

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Embodiment 1

[0042] The present embodiment provides the method that adopts sol-gel method to prepare mesoporous bioactive glass, specifically:

[0043] Premix 30mL deionized water and 120mL nitric acid solution with a concentration of 2M, then add 3g of P123 surfactant to dissolve, adjust the pH value of the solution to below 1 with nitric acid, add 8.5g tetraethyl orthosilicate (TEOS), 0.98 g triethyl phosphate (TEP) and 5.94 g calcium nitrate, stirred at 60°C for 48 hours, dried in an oven at 100°C for 12h, and the obtained powder was calcined in a muffle furnace at 600°C for 6h, with a heating rate of 1°C / min, the obtained product is mesoporous bioactive glass.

Embodiment 2

[0045] This embodiment provides a preparation method of bioactive glass / biomimetic functional polypeptide complex, specifically:

[0046] Add 50 mg of mesoporous bioactive glass to 10 mL of PBS buffer, ultrasonically disperse for 10 minutes, add 15 mg of QP5 polypeptide (its amino acid sequence is shown in SEQ ID NO.1), stir magnetically at 4°C for 24 h, centrifuge, and wash the precipitate with PBS Three times, vacuum freeze-dry. The mesoporous bioactive glass described in this example was prepared according to the method of Example 1.

[0047] The bioactive glass / biomimetic functional polypeptide complex prepared in this example is subjected to infrared analysis, and its infrared spectrum is as attached figure 1 , where MBG means bioactive glass, QP5 means biomimetic functional polypeptide, and MBG-Q means bioactive glass / biomimetic functional polypeptide complex. The results showed that, compared with the bioactive glass not loaded with polypeptide, the bioactive glass / bi...

Embodiment 3

[0049] This example provides an investigation of different dosage ratios of bioactive glass / biomimetic functional polypeptide complexes, specifically:

[0050] Add 50mg of mesoporous bioactive glass to 10mL of PBS buffer, ultrasonically disperse it for 10 minutes, then add QP5 polypeptides of different quality to suspend 50mg of bioactive glass powder in 10mL of different concentrations (0.2-7.0mg / mL) In the peptide solution, stir magnetically at 4°C for 24 hours, centrifuge, collect the supernatant, measure the concentration of unloaded free peptide in the supernatant with a UV-visible spectrophotometer, and calculate the concentration of the peptide encapsulated into the bioactive glass carrier. The encapsulation efficiency of the polypeptide and the drug loading capacity of the complex.

[0051] The encapsulation efficiency calculation formula is as follows: EE=(C0-C1) / C0×100%;

[0052] Among them, EE represents the encapsulation efficiency, CO represents the total concent...

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Abstract

The invention discloses a bioactive glass / bionic functional polypeptide complex as well as a preparation method and application thereof, belongs to the technical field of biology, and solves the technical problems that in the prior art, a bionic mineralization functional polypeptide gel preparation lacks a calcium and phosphorus source necessary for remineralization, and cannot safely retain the vitality of a dental pulp and dentin complex and induce dentin regeneration. The bioactive glass / bionic functional polypeptide complex takes mesoporous bioactive glass as a carrier to load a bionic functional polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The preparation method comprises the following steps: adding the mesoporous bioactive glass into a buffer solution, dispersing, adding the bionic functional polypeptide, uniformly stirring, carrying out solid-liquid separation, and cleaning and drying the obtained solid, thereby obtaining the bioactive glass / bionic functional polypeptide complex. The bioactive glass / bionic functional polypeptide complex disclosed by the invention not only has a bionic mineralization effect on hard tissues, but also can provide a calcium and phosphorus ion source required by mineralization, further has a mineralization promoting effect on dental pulp cells, and can further promote dentin repair.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the fields of dental caries prevention technology and dentin regeneration, and specifically relates to a bioactive glass / bionic functional polypeptide complex, a preparation method and an application. Background technique [0002] Caries and pulp disease are common clinical oral diseases. Dentin is the hard tissue that constitutes the main body of the tooth, which is secreted and mineralized by odontoblasts. Dentin surrounds the pulp cavity and contains pulp tissue. Odontoblasts, pulp cells, and dental pulp stem cells with self-renewal and multilineage differentiation potential exist in dental pulp. Under external stimuli such as caries and dental trauma, the dentin can be destroyed, and then the pulp can be damaged. [0003] Currently, the classic treatment is removal of the affected tooth tissue and its subsequent replacement with artificial filling materials. However, in the case of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/06A61K47/02A61P1/02
CPCA61K38/1709A61K47/02A61K9/06A61P1/02
Inventor 张凌琳陆君卓刘珍琪王琨陈相书
Owner SICHUAN UNIV
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