SARS-CoV-2 detection kit based on indirect method
A detection kit, sars-cov-2 technology, applied in the field of biomedicine, can solve the problems of limitations, unsatisfactory antibody sensitivity, etc., achieve high specificity and sensitivity, save operation time and cost, and high detection sensitivity Effect
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Embodiment 1
[0083] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:
[0084] 1.1 R1 magnetic bead components:
[0085] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:
[0086] ① Anti-new coronavirus N protein mouse monoclonal antibody;
[0087] ②SARS-CoV-2 Spike protein S1 subunit;
[0088] ③SARS-CoV-2 Spike protein S2 subunit;
[0089]④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;
[0090] ⑤SARS-CoV-2 M protein
[0091] ⑥SARS-CoV-2 E protein
[0092] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recombin...
Embodiment 2
[0101] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:
[0102] 1.1 R1 magnetic bead components:
[0103] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:
[0104] ① Anti-new coronavirus N protein mouse monoclonal antibody;
[0105] ②SARS-CoV-2 Spike protein S1 subunit;
[0106] ③SARS-CoV-2 Spike protein S2 subunit;
[0107] ④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;
[0108] ⑤SARS-CoV-2 M protein
[0109] ⑥SARS-CoV-2 E protein
[0110] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recombi...
Embodiment 3
[0119] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:
[0120] 1.1 R1 magnetic bead components:
[0121] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:
[0122] ① Anti-new coronavirus N protein mouse monoclonal antibody;
[0123] ②S1 subunit of SARS-CoV-2 Spike protein;
[0124] ③ SARS-CoV-2 Spike protein S2 subunit;
[0125] ④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;
[0126] ⑤SARS-CoV-2M protein
[0127] ⑥SARS-CoV-2E protein
[0128] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recom...
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