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SARS-CoV-2 detection kit based on indirect method

A detection kit, sars-cov-2 technology, applied in the field of biomedicine, can solve the problems of limitations, unsatisfactory antibody sensitivity, etc., achieve high specificity and sensitivity, save operation time and cost, and high detection sensitivity Effect

Active Publication Date: 2021-06-15
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the "Diagnosis and Treatment Program for Novel Coronavirus Pneumonia (Trial Sixth Edition)", the current method of confirming the diagnosis of COVID-19 is to use real-time fluorescent RT-PCR to detect the positive nucleic acid of the new coronavirus, or to sequence the virus gene. The problem of missed detection of detection reagents has been repeatedly exposed, and the positive detection rate is only 30-50%, mainly due to the limitations of sampling methods
[0008] However, at present, the sensitivity of antibody detection is still not satisfactory.

Method used

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  • SARS-CoV-2 detection kit based on indirect method
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  • SARS-CoV-2 detection kit based on indirect method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0084] 1.1 R1 magnetic bead components:

[0085] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0086] ① Anti-new coronavirus N protein mouse monoclonal antibody;

[0087] ②SARS-CoV-2 Spike protein S1 subunit;

[0088] ③SARS-CoV-2 Spike protein S2 subunit;

[0089]④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0090] ⑤SARS-CoV-2 M protein

[0091] ⑥SARS-CoV-2 E protein

[0092] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recombin...

Embodiment 2

[0101] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0102] 1.1 R1 magnetic bead components:

[0103] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0104] ① Anti-new coronavirus N protein mouse monoclonal antibody;

[0105] ②SARS-CoV-2 Spike protein S1 subunit;

[0106] ③SARS-CoV-2 Spike protein S2 subunit;

[0107] ④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0108] ⑤SARS-CoV-2 M protein

[0109] ⑥SARS-CoV-2 E protein

[0110] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recombi...

Embodiment 3

[0119] This kit is mainly divided into calibrator and detection reagent, among which, the detection reagent consists of 3 parts:

[0120] 1.1 R1 magnetic bead components:

[0121] Composed of 6 kinds of paramagnetic magnetic beads and magnetic bead diluent, the combination of magnetic bead coating protein is as follows:

[0122] ① Anti-new coronavirus N protein mouse monoclonal antibody;

[0123] ②S1 subunit of SARS-CoV-2 Spike protein;

[0124] ③ SARS-CoV-2 Spike protein S2 subunit;

[0125] ④ SARS-CoV-2 Spike protein S1 subunit receptor binding domain (Receptor Binding Domian, RBD) protein;

[0126] ⑤SARS-CoV-2M protein

[0127] ⑥SARS-CoV-2E protein

[0128] Among them, the antiviral N protein mouse monoclonal antibody can be extracted from mouse ascites or hybridoma cells cultured in vitro, and can be a variety of monoclonal antibodies targeting different immune sites of different N proteins; S1, S2, RBD, M, and E proteins can be extracted naturally or expressed recom...

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Abstract

The invention relates to the field of biological medicine, in particular to an SARS-CoV-2 detection kit based on an indirect method. The kit comprises (a) a solid phase conjugate. The solid-phase conjugate consists of a solid-phase carrier as well as an antigen and an antibody which coat the solid-phase carrier. The antibody is a monoclonal antibody for resisting novel coronavirus nucleocapsid N protein. The antigen comprises an S1 subunit, an S2 subunit, an RBD, an N protein and an E protein of the SAR-CoV-2 Spike protein. The kit further comprises (b) an anti-antibody and an antibody marked with a chemiluminescent marker. The antibody is a monoclonal antibody to the novel coronavirus nucleocapsid N protein, but is bound to a different epitope from the antibody in (a). The anti-antibody is at least one of an anti-human IgG secondary antibody, an anti-human IgA secondary antibody and an anti-human IgM secondary antibody. The anti-antibody is a monoclonal antibody or a polyclonal antibody.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a SARS-CoV-2 detection kit based on an indirect method. Background technique [0002] The new type of coronavirus (SARS-CoV-2) belongs to the genus β coronavirus, the particles are round or oval, with a diameter of 60nm-140nm, and compared with SARS, it has a higher infectivity. The new coronavirus has a strong ability to spread from person to person, and the crowd is generally susceptible. Patients are the most important source of infection. Therefore, early detection, early isolation, and early treatment of asymptomatic infections, suspected and confirmed cases are still the next epidemic situation. The main line of prevention and control. How to accurately detect the virus, quickly identify patients with new crown infection, and reduce cross-infection; and how to effectively monitor the disease process and antibody production will be the focus of the battle against the epidemic. B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/58G01N33/543
CPCG01N33/56983G01N33/6854G01N33/58G01N33/543G01N2333/165
Inventor 钱纯亘汪大明程方明龚育清吴力强胡鹍辉
Owner SHENZHEN YHLO BIOTECH
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