Screening method and screening system for homozygous mutation seeds in gene mutation materials

Abstract

The invention provides a screening method and a screening system for homozygous mutation seeds in gene mutation materials, and belongs to the field of tobacco seedling culture. The screening method for the homozygous mutation seeds comprises the following steps: S1, inquiring storage information of mutation materials to be screened; s2, screening out seeds which are not dibbled; s3, removing the seeds which are not subjected to gene mutation, and screening out the seeds which are not dibbled and are subjected to gene mutation; s4, homozygote seeds which are not dibbled and subjected to gene mutation are screened out; and S5, screening out seeds with specific genes from the homozygous mutation seeds. According to the screening method, specific gene seeds can be effectively screened out according to requirements; meanwhile, the seeds can be quickly, efficiently and accurately screened out by adopting the screening system. Comprising a seed storage information base module, a seed query module, an undibbling seed screening module, a gene mutation seed screening module, a homozygote mutation seed screening module and a specific gene seed screening module.

Description

technical field [0001] The invention belongs to the field of tobacco seedling cultivation, and in particular relates to a screening method and a screening system for homozygous mutant seeds in gene mutation materials. Background technique [0002] A mutant is a material with a heritable mutation of a certain trait or a mutation of a certain gene, which can be obtained through spontaneous mutation and induced mutation. Spontaneous mutation refers to the occurrence of genetic variation or mutation in the natural environment, but the mutation rate is low, generally not exceeding 0.1%. Tobacco super mutants are spontaneous mutations. Induced mutation is through human intervention, using physical, chemical and biological means to cause a large number of mutations in individual DNA in a short period of time, with a high mutation rate of about 3%. The creation of a large number of mutants mainly relies on the method of induced mutagenesis. [0003] With the maturity of mutation ...

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Problems solved by technology

[0003] With the maturity of mutation technology, thousands of mutant materials are gradually produced, and each mutant material corresponds to a different gene type. Therefore, it is necessary to screen homozygous mutant seeds from thousands of mutant materials

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