Application of metabonomics analysis technology to identification of Danxiang Dancong tea

A technology of metabolomics and Dancong tea, which is applied in the field of oolong tea identification, can solve the problems that cannot be applied and provide technical guidance

Active Publication Date: 2021-07-16
广东省农业科学院农业质量标准与监测技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the non-targeted metabolic analysis and detection method of...

Method used

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  • Application of metabonomics analysis technology to identification of Danxiang Dancong tea
  • Application of metabonomics analysis technology to identification of Danxiang Dancong tea
  • Application of metabonomics analysis technology to identification of Danxiang Dancong tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A Metabolic Analysis Method for Identifying Duck Squid Wild Tea Characteristics:

[0051] 1) Sample preparation

[0052] Choose tea variety for duck fragrance, big black leaves, yellow branch, honeylanca, Zhulangxiang, Ling head single plea tea and Fujian's iron goge, including Tieguanyin, other six kinds of small tea varieties; Each variety collected 6 finished tea, and all sample acquisitions set three repetitions.

[0053] The chopped tea is uniform sampled by 0.0 ° C is preserved in the -20 ° C refrigerator after 60 mesh sieve. Weighing 100 mg tea powder samples in a 1.5 ml centrifuge tube, 1 ml of methanol / water containing 1% formic acid (75 / 25, V / V) is added; the mixture is ultrasound for 10 min under 20 ° C, and then low temperature centrifuge 11000 rpm was centrifuged at 4 ° C for 10 min; taking the supernatant, a 0.22 μm organic filtramine in a 2 ml injection vial, that is, the tea extract is obtained. The isometric solution is taken from each sample solution, ...

Embodiment 2

[0074] A metabolic analysis method identified by a single plexus characteristic component of a duck fragrant, which is the same as that of the specific steps of Example 1, and the difference is that the sample amount and the extractant are different, specifically at the end of the tea powder at 50 ml of centrifuge tube. In addition, 25 ml of hot water is added.

[0075] Such as Figure 6 As shown, the above and the following diagrams are respectively positive and negative ion mode, respectively, compared with the predecessor method of Example 1, the characteristic peak separation effect of the total ion graph obtained in Example 2 is poor, partially characterized The response is less than in Example 1, especially in the negative ion mode is not as good as in Example 1.

Embodiment 3

[0077] A metabolic syndrome analysis method identified for the characteristics of the duck fragrant monarch, which is the same as the specific steps of the first embodiment, and the difference between the source data of the screeing difference is to select all the extract or the two MzVault or MzCloud. Level mass spectrometry can be achieved more than 70% of metabolites.

[0078] Data analysis of metabolites with a score greater than 70: The six difference metabolites were selected for the characteristic marker of the duck, and the core of the marker was described in Table 2, and the tea sample was determined by the metabolic component analysis method. The peak area of ​​the above compounds. Compare the results of the peak area of ​​the compound in the ducks, view |Log 2 Fold ChanGe| The value is greater than 1.5, which can be used to discriminate whether it is a duck fragrance.

[0079] As will be described in Table 2, Example 3 reduces the number of feature markers, and 6 compou...

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Abstract

The invention belongs to the technical field of oolong tea identification, and relates to application of a metabonomics analysis technology to identification of Danxiang Dancong tea. In the metabonomics analysis technology, characteristic components of the Danxiang Dancong tea are identified through Log2 Fold Change, adj.p and VIP values, and the screening conditions are |Log2 Fold Change|>1.5, adj.p<0.01, and VIP is greater than 1. The metabonomics analysis technology is utilized to establish a comprehensive and unambiguous non-targeted metabonomics analysis method of the duck's droppings, metabolic component analysis can be performed on different varieties of tea leaves, and characteristic components of the duck's droppings can be accurately found out; the metabonomics analysis method of the characteristic components of the Danxiangxiang Dancong tea is established on the basis of the chemical components of the tea leaves, and the characteristic markers of the Danxiangxiang beneficial to true and false identification, geographical traceability, high-quality variety screening, flavor regulation and the like of the Danxiangxiang are screened from big data in combination with chemometrics analysis.

Description

Technical field [0001] The present invention belongs to the technical field of oolong tea appraisal, and specific to a metabolic syndiotics analysis technology identification of duck shafts. Background technique [0002] Ducks incense singles (hereinafter referred to as "Ducks") is a high-value variety of special charm in Phoenix Single bush tea, whose leaves are like large black leaves, fragrant flavors, tastes and fragrance Ok, you are free to love consumers. Although it is the largest variety of local plants, it is still in short supply, and the price of different varieties, grades of tea is different. Due to economic efficiency, some unscrupulous traders are full of fake and chaos. [0003] The traditional identification method is to evaluate the characteristics of tea through the sensory review, which has strong subjectivity. The chemical composition of tea determines the drinking value and characteristics of tea, and establishes the metabolic syndrome of the tea characteris...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634
Inventor 赵洁刘香香陈岩刘雯雯张欣王旭王富华
Owner 广东省农业科学院农业质量标准与监测技术研究所
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