Method and device for detecting concentration of salmonella in food based on micro-fluidic chip

Abstract

The invention discloses a method and a device for detecting the concentration of salmonella in food based on a micro-fluidic chip, and the device comprises a micro-fluidic pipeline area, a silk-screen printing electrode layer, an electronic refrigeration sheet layer, a bimorph layer and a PCB layer from top to bottom, a sample inlet, a carbonic acid liquid storage chamber, an ethanol liquid storage chamber, a gold nanoparticle solution storage chamber, a lipopolysaccharide antibody solution chamber, a BSA solution storage chamber and a PBS buffer solution storage chamber are respectively arranged on the glass substrate at the bottom of the micro-fluidic pipeline area; the sample inlet is sequentially connected in series with a first micro-mixing cavity, a second micro-mixing cavity and a first through hole through a pipeline; the method comprises the following steps: modifying a screen-printed electrode, measuring a first impedance value of the surface of a working electrode, dropping a salmonella sample liquid into the sample inlet, dropping the liquid into the surface of a working area, measuring a second impedance value of the surface of the working electrode, comparing the two impedance values with a relational graph, and simulating to obtain a salmonella concentration value. And the multi-pipeline micro-fluidic chip is adopted, so that the detection is quick, simple and convenient, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of biomedical detection, in particular to a detection technology for special Salmonella based on a microfluidic chip, which is used for detecting the concentration of Salmonella in food. Background technique [0002] Salmonella is a common food-borne pathogen. Among the types of bacterial food poisoning, food poisoning caused by Salmonella ranks first. The pathogen of salmonellosis belongs to Enterobacteriaceae, Gram-negative enterobacteriaceae, which can survive for several months in food such as water, milk and meat, and infection with Salmonella can easily cause typhoid fever, infectious diarrhea, enteric fever, gastroenteritis, etc. disease. Therefore, a portable device is needed to detect the concentration of Salmonella in food simply and quickly. [0003] At present, the detection of Salmonella has always been based on traditional detection methods, using conventional methods such as non-selective and selectiv...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems existing in the existing detection technology of Salmonella in food, and propose a detection device and method for the concentration of Salmonella in food based on a microfluidic chip, which adopts a multi-pipeline microfluidic chip to detect quickly and easily with high sensitivity. high

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