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Inoculation method for shortening rapid propagation growth cycle of phalaenopsis

A growth cycle, Phalaenopsis technology, applied in the field of inoculation to shorten the rapid propagation growth cycle of Phalaenopsis, can solve the problems of increased production cost, increased production capacity, bud variation, etc.

Pending Publication Date: 2021-08-03
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the existing rapid propagation can obtain enough buds, the method has the following defects: 1. A large amount of cytokinins are needed in the medium, which will cause the buds to vary in the later growth after the amount of cytokinins is large Risk; 2. The cultivation cycle of this method is more than 45 days, and the long cycle will lead to increased production costs and is not conducive to the increase of production capacity

Method used

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  • Inoculation method for shortening rapid propagation growth cycle of phalaenopsis
  • Inoculation method for shortening rapid propagation growth cycle of phalaenopsis
  • Inoculation method for shortening rapid propagation growth cycle of phalaenopsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The strong buds are cut and inoculated, and the strong buds meet the following conditions: the leaves are dark green, the stems and leaves in the lateral buds are differentiated, and the stems are thick;

[0031] Cut along the junction of the topmost blade and stem of the strong buds, remove the terminal buds from the strong buds, and insert 1 strong bud into the culture medium for 21 days. The insertion depth in the medium was all 0.20 of the bud height. The culture conditions are: the light is 1800lux, the average temperature is 25°C, the light is 12 hours a day, and the light time is from 7:00 to 19:00; the components and contents of the medium are as follows: the weight ratio is 100:10:4:0.2:1.5 MS medium, mashed potatoes, mashed bananas, ascorbic acid and corn juice.

[0032] See the results of the training figure 1 .

Embodiment 2

[0034] Weak buds are cut and inoculated, and the weak buds meet the following conditions: the leaves are light green or white buds, and the stems and leaves in the lateral buds are undifferentiated;

[0035] Keep the terminal buds of the weak buds, separate the weak buds with a straight knife along the bud gap, and insert 2 weak buds into the medium for cultivation for 21 days. The insertion depth of the weak buds in the medium is equal to that of bud 0.20 of the body height. The culture conditions are: the light is 1800lux, the temperature is 25°C, the light is 12 hours a day, and the light time is 7:00-19:00; the components and contents of the medium are as follows: MS with a weight ratio of 100:10:4:0.2:1.5 Culture medium, mashed potatoes, mashed bananas, ascorbic acid and corn juice.

[0036] See the results of the training figure 2 .

Embodiment 3

[0038] According to the method of Example 1, the difference is that the insertion depth of the strong buds in the culture medium is 0.15 of the height of the bud body.

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Abstract

The invention discloses an inoculation method for shortening the rapid propagation growth cycle of phalaenopsis. A phalaenopsis bud contains at least one of strong buds and weak buds, the strong buds meet the following conditions: the leaf color is dark green, lateral buds, middle stems and leaves are differentiated; and the weak buds meet the following conditions: the leaf color is light green or buds are white, and middle stems and leaves of lateral buds are not differentiated; and the stems of the strong buds are thicker than those of the weak buds. The method comprises at least one of strong bud cutting inoculation and weak bud cutting inoculation. The method of strong bud cutting inoculation comprises the following steps: removing terminal buds from the strong buds, and then inserting the strong buds into a first culture medium for culturing by taking one strong bud as a unit. The method of weak bud cutting inoculation comprises the following steps: reserving terminal buds of the weak buds, separating the weak buds by a straight knife along bud seams, and inserting the weak buds into a second culture medium by taking 2-3 weak buds as units for culturing, wherein the insertion depth of the strong buds and the insertion depth of the weak buds in the culture mediums are both 0.15-0.25 of the height of the buds. The method can shorten the rapid propagation cycle.

Description

technical field [0001] The invention relates to rapid propagation of Phalaenopsis, in particular to an inoculation method for shortening the growth period of rapid propagation of Phalaenopsis. Background technique [0002] In the process of rapid propagation of Phalaenopsis asexual reproduction, the goal of rapid propagation is to obtain enough buds as soon as possible. Currently, the division and proliferation of buds is used to achieve rapid propagation. The method generally adopted for the rapid propagation of existing buds is: remove the dominance of terminal buds, and culture them with MS medium containing the number of cell divisions in units of 1-2. [0003] Although the existing rapid propagation can obtain enough buds, there are following defects in this method: 1. A large amount of cytokinins need to be used in the culture medium, and large amount of cytokinins will cause the buds to vary in the later growth Risk; 2. The cultivation cycle of this method is more th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 汪锡文邱许凤周静伟章明紫裴宝平张晓斌黄立斌颜丽魏玉龙赵帮秦
Owner 芜湖东源新农村开发股份有限公司
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