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A kind of tissue culture method of wild mountain peach thick plum

A technique of tissue culture and Prunus chinensis, applied in the field of plant tissue culture, can solve the problems of unsystematic research on tissue culture of Prunus chinensis, difficulty in preserving the original female parent, and high cost of seedlings, so as to maintain stress resistance and ornamental quality Sexuality, good stress resistance and ornamental properties, and the effect of shortening the seedling breeding cycle

Inactive Publication Date: 2017-07-07
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In 2009, cutting propagation was carried out in the nursery of Heilongjiang Forestry Research Institute in Harbin City, Heilongjiang Province, and the survival rate was very low.
Therefore, another seed propagation experiment was carried out, and the survival rate was relatively high, but the emergence rate was still very low, and the cost of seedlings was very high
Moreover, sexual reproduction is difficult to preserve the excellent characteristics of the original mother
At present in China, there is no systematic research on the tissue culture of wild Prunus chinensis in the forest area of ​​Heilongjiang Province

Method used

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  • A kind of tissue culture method of wild mountain peach thick plum
  • A kind of tissue culture method of wild mountain peach thick plum
  • A kind of tissue culture method of wild mountain peach thick plum

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specific Embodiment approach 1

[0021] Specific embodiment one: a kind of method for the tissue culture of wild Prunus chinensis of the present embodiment, it is to carry out according to the following steps:

[0022] One, the selection of explants: the explants select the wild Prunus chinensis plant;

[0023] 2. Disinfection of explants: take the lateral buds with terminal buds as explants and cut them into 4-5cm bud-bearing stem segments, clean them with washing powder, and then rinse them with clean water for 1-2 hours; place them on an ultra-clean workbench Soak in 75% ethanol by volume for 30 seconds, rinse with sterile water for 3 times, air-dry on sterile filter paper, then sterilize with 0.1% by mass mercuric chloride for 8 minutes, and then rinse with sterile water for 3 Second-rate;

[0024] 3. Primary culture: cut the sterilized explants into stems of about 1 cm and insert them into the primary medium for culture. The culture conditions are: the culture temperature is 20-24°C, the light intensity...

specific Embodiment approach 2

[0033]Specific embodiment two: what this embodiment is different from specific embodiment one is: selecting the wild Prunus chinensis plant in the step 1 refers to: selecting the side bud stem section of the band terminal bud that germinated in the same year as the Prunus persicae plant in the wild, and collecting it in the field The Shantao thick plum plant is stored in a low-temperature incubator for subsequent use. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Specific embodiment three: the difference between this embodiment and specific embodiment one is: explant disinfection in step 2: remove the unused part of the collected twigs, cut them into 4-5 cm bud stem segments, and place them in a washing machine In a beaker of powdered water, cover the mouth of the beaker with a gauze net, shake the beaker with your hands for 5 minutes, and rinse the beaker with running water for 1 to 2 hours. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for tissue culture of wild Prunus persicae, which relates to a method for tissue culture of Prunus persicae. The invention aims to solve the problem of poor effect of the tissue culture method of wild mountain peach thick plum. The method of the present invention comprises: 1) selection of explants, 2) disinfection of explants, 3) primary culture, 4) proliferation and cultivation of adventitious buds, 5) strong seedling cultivation, 6) rooting cultivation. By adopting the method of the invention, the germination rate of side buds with terminal buds reaches as high as 82.8 percent, the aseptic germination rate reaches 61.3 percent, the multiplication coefficient reaches 4.89, and the rooting rate reaches 91.2 percent. The method of the present invention can better keep the stress resistance and ornamental properties of the female parent, greatly shortens the seedling cultivation period, can obtain rooted seedlings in 5 months, and effectively improves the reproduction coefficient of the thick plum; compared with seed propagation, Tissue culture can effectively provide low-cost and highly ornamental green seedlings, which can effectively reduce the cost of seedlings and save expenses.

Description

technical field [0001] The invention belongs to a plant tissue culture method, in particular to a wild mountain peach thick plum tissue culture method. Background technique [0002] Prunus chinensis is called Prunus variegata (scientific name: Padusmaackii (Rupr.) Kom) small tree, 4-10m high. Leaves are elliptic, diamond-shaped ovate, or oblong-obovate rarely, 4-8cm long, with irregular glandular sharp serrations on the leaf margin, pubescent on the back along the midrib, covered with purple-brown glands. Raceme, base without leaves. Flowers white, densely packed; styles and stamens nearly equal in length. Fruit nearly spherical, 5-7mm in diameter, purple-brown. The flowering period is April-May, and the fruiting period is June-October. It grows well on moist and fertile sandy loam soil, has strong germination ability and less pests and diseases. Sun-loving and shade-tolerant, strong cold resistance, afraid of waterlogging, intolerant of drought and barrenness, grows we...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 舒钰李晶邢亚娟王丹蒙宽宏王承义赵学丽
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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