Streptococcus thermophilus for producing gamma-aminobutyric acid and application of streptococcus thermophilus
The technology of Streptococcus thermophilus and aminobutyric acid is applied in the field of microbial fermentation engineering, which can solve the problems of high synthesis amount of γ-aminobutyric acid, low synthesis amount of γ-aminobutyric acid, short fermentation time and the like, and achieves good taste, Easy to operate, high application value effect
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Embodiment 1
[0102] In this embodiment, the bacterial strains producing γ-aminobutyric acid are screened, and the specific steps are as follows:
[0103] (1) Milk tofu samples were diluted with normal saline at 10-8, 10-9, and 10-10 gradients, and spread on the surface of MRS solid medium containing bromocresol purple, cultured at 37°C for 60 hours, and screened A single yellow colony was obtained; the resulting single colony was streaked and cultured three times to obtain pure colonies, inoculated in MRS liquid medium, cultured at 37°C for 18 hours, mixed with 40% glycerol, and stored at -80°C cryopreservation;
[0104] (2) Inoculate the bacterium of described cryopreservation in MRS liquid culture medium, at 37 ℃, activate culture 18h, activate 2 times like this, inoculate in containing 1% L-sodium glutamate by 2% inoculation volume In the MRS liquid culture medium, at 37° C., statically culture for 48 hours, and centrifuge at 6500 rpm for 5 minutes to obtain the culture supernatant. U...
Embodiment 2
[0108] In this example, morphological identification and molecular biological identification of 16SrRNA were carried out on the γ-aminobutyric acid-producing strain screened in Example 1. The specific steps are as follows:
[0109] (1) Morphological identification:
[0110] The strains stored at -80°C were taken out, thawed and directly streaked on the MRS solid medium plate, cultured at 37°C for 48 hours, and the shape, color, size, transparency, edge and surface of the colony were observed. Pick a single colony for Gram staining. After staining, use a Motic digital biological microscope to observe the size and shape of the cells under an oil lens (100×), and take pictures to record. The results are as follows: figure 1 shown.
[0111] It can be seen from the observation that the colonies of the screened strains on the MRS medium are small, round, milky white, and smooth in surface; as can be seen from the figure, under the microscope, the bacteria are in the shape of curved...
Embodiment 3
[0118] Based on the results of morphological identification and 16S rRNA molecular biological identification in Example 2, it was confirmed that the strain belonged to Streptococcus thermophilus and named Streptococcus thermophilus ST36. Streptococcus thermophilus ST36 was deposited at the Institute of Microbiology, Chinese Academy of Sciences (CGMCC) on September 18, 2020. The address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, postcode 100101, and the deposit number is CGMCCNO. 20676.
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