Myeloblastoma cell exosome capable of inducing polarization of M2-like TAMs

A technology of medulloblastoma and exosomes, applied in the field of tumor

Pending Publication Date: 2021-08-24
PEKING UNIV THIRD HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a medulloblastoma cell exosome microRNA that can induce the polarization of M2-like TAMs, to solve the problem of whether exosomes mediate the transfer of inflammation-related microRNA medulloblastoma cells to TAMs

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  • Myeloblastoma cell exosome capable of inducing polarization of M2-like TAMs
  • Myeloblastoma cell exosome capable of inducing polarization of M2-like TAMs
  • Myeloblastoma cell exosome capable of inducing polarization of M2-like TAMs

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Embodiment Construction

[0034] The technical solution of the present invention is described in detail below through the examples, and the following examples are only exemplary and can only be used to explain and illustrate the technical solution of the present invention, rather than being interpreted as a limitation to the technical solution of the present invention.

[0035] In the following examples of the present application, the reagents, materials, instruments, etc. used, unless otherwise specified, can be purchased commercially.

[0036] In order to verify that SHH-type MB cell exosomes induced TAMs polarization, SHH-type MB cell exosomes were isolated and purified from Daoy cell culture supernatant by differential centrifugation and ultracentrifugation, and used to treat macrophages and a. Exosomes were first identified. Transmission electron microscopy showed that the isolated extracellular vesicles had a typical double-membrane structure and a cup-shaped morphology, with a diameter of about...

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Abstract

The invention relates to a medulloblastoma cell exosome capable of inducing polarization of M2-like TAMs. The exosome comprises a combination of let-7i-5p and miR-221-3p. Less let-7i-5p and miR-221-3p are expressed in the SHH type MB cell exosome, the inhibitory effect on PPARG is smaller than that of other molecular subtypes, and the activation capacity on an NF-kB signal channel at the downstream of PPARG is correspondingly weaker than that of other molecular subtypes, so that the SHH type MB cell exosome can better promote TAMs to be polarized to M2 samples; a combined administration experiment of a PPARG antagonist and an SMO inhibitor is further carried out, and it is found that the PPARG antagonist can significantly inhibit polarization of TAMs to M2 in a mouse tumor microenvironment, so that TAMs are activated to M1, and the anti-tumor activity of the SMO inhibitor is significantly enhanced.

Description

technical field [0001] The invention belongs to the technical field of tumors, and in particular relates to a medulloblastoma cell exosome capable of inducing the polarization of M2-like TAMs. Background technique [0002] Medulloblastoma (MB) is the most common malignant brain tumor in children, accounting for about 20% of central nervous system tumors in children. It is classified as WHO grade IV and is very prone to metastasis and recurrence. Survivors are often accompanied by multiple sequelae. The tumor is highly heterogeneous, and there are currently four recognized molecular subtypes: WNT type, SHH type, Group 3 type, and Group 4 type. Among them, the Sonic Hedgehog (SHH) subtype is associated with abnormal activation of the Shh signaling pathway, accounting for about 30% of the total cases. [0003] Tumor microenvironment (TME) is a complex system composed of tumor cells, a variety of non-cancer cells, cytokines, matrix components, blood and lymphatic circulation, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/113C12Q1/6888
CPCC12N5/0693C12N15/113C12N2310/141C12Q1/6888C12Q2600/158C12Q2600/178
Inventor 常青朱靓怡杨迎
Owner PEKING UNIV THIRD HOSPITAL
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