Plant tissue culture method by adding culture medium under bacterial environment condition

A technology for plant tissue culture and environmental conditions, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of limited popularization and application, high investment and technical requirements, high production costs, etc., to reduce costs and simplify cultivation Process and difficulty, effect of promoting plant growth

Active Publication Date: 2021-09-03
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strict aseptic operation and culture environment, traditional tissue culture requires high investment and technical requirements, resulting in high production costs, which greatly limits the popularization and application of this technology

Method used

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  • Plant tissue culture method by adding culture medium under bacterial environment condition
  • Plant tissue culture method by adding culture medium under bacterial environment condition
  • Plant tissue culture method by adding culture medium under bacterial environment condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The incubator such as Figure 1~3 As shown, it includes a detachable box body (1) and a cover body (2); a seedling tray (5) is placed in the box body (1), and the plastic box main body (1) is 25-35 centimeters long and wide. 18-23 centimeters, 13-15 centimeters high, 0.2-0.3 centimeters from the bottom of the box is provided with an air inlet (4) with a diameter of 0.3-0.5 centimeters; the plastic box cover is 25-35 centimeters long and 18-23 centimeters wide , 1.5-3.0 cm high, with a 0.8-1.0 diameter ventilation hole (3) on the left and right sides, and the cover and the main body are buckled and sealed;

[0032] The length and width of the seedling-carrying tray (5) are smaller than the main body of the plastic box, and the tray has 8-12 holes (6), the diameter of the hole (6) is 5-6 cm, and the diameter of the hole (6) is 2-6 cm. 3 cm, 5-6 cm deep;

Embodiment 2

[0034] (1) prepare the incubator for plant tissue culture;

[0035] (2) After the cultivation incubator is sterilized, put the seedling-carrying tray in the cultivation box, and place it under sterile conditions for standby;

[0036] (3) sugarcane proliferation seedling cluster buds are divided into small cluster seedlings of 2-3 strains, the small cluster seedlings are put into the hole of the seedling-carrying hole of the box main body, add MS medium, cover the cover body, and Continuously inject sterile air into the air inlet for cultivation;

[0037] (4) Add proliferation medium after culturing for 15 days;

[0038] (5) After the hole is full of tissue culture seedlings, add rooting medium to carry out rooting cultivation;

[0039] (6) After the tissue-cultured seedlings take root, remove the culture box for pseudo-planting, and stop feeding sterile air into the culture box.

[0040] The method of adding the proliferation medium described in step (4) and the rooting med...

Embodiment 3

[0054] (1) prepare the incubator for plant tissue culture;

[0055] (2) After the cultivation incubator is sterilized, put the seedling-carrying tray in the cultivation box, and place it under sterile conditions for standby;

[0056] (3) sugarcane proliferation seedling cluster buds are divided into small cluster seedlings of 2-3 strains, the small cluster seedlings are put into the hole of the seedling-carrying hole of the box main body, add MS medium, cover the cover body, and Continuously inject sterile air into the air inlet for cultivation;

[0057] (4) adding proliferation medium after culturing for 20 days;

[0058] (5) After the hole is full of tissue culture seedlings, add rooting medium to carry out rooting cultivation;

[0059] (6) After the tissue-cultured seedlings take root, remove the culture box for pseudo-planting, and stop feeding sterile air into the culture box.

[0060] The method of adding the proliferation medium described in step (4) and the rooting ...

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Abstract

The invention relates to the technical field of biotechnology, and particularly discloses a plant tissue culture method by adding a culture medium under a bacterial environment condition. According to the method, one-way air flow is formed in a culture box, a proliferation culture medium and a rooting culture medium containing a bacteriostatic agent solution are added through a medical injector, and the bacteriostatic agent solution is prepared from fresh iris ensata roots, fresh banana rhizomes, fresh sophora subprostrata roots, fresh Scirpus triangulatus roots, fresh bitter gourd rhizomes and distilled water. When the method is used for plant tissue culture, the operation of adding the culture media under the bacterial environment condition can be realized, the risk of infecting germs in the plant tissue culture process is low, and the rooting rate and transplanting survival rate of cultured tissue culture seedlings are high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for plant tissue culture by adding culture medium under the condition of bacteria environment. Background technique [0002] Tissue cultivation is one of the most widely used plant asexual reproduction techniques. This technique is not limited by the environment and seasons. The traits of the offspring are completely consistent with the female parent. The growth cycle of tissue culture seedlings is short and the area of ​​the ear picking nursery is small. It can develop high-quality and high-yield forest tree seedling production in a limited time and space, promote the preservation, utilization and promotion of good germplasm resources, and is suitable for large-scale production. Therefore, it has a wide range of applications in flowers, fruit trees and sugarcane. application. Due to the strict aseptic operation and culture environment, traditional tissue cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N33/12A01N59/08A01N47/28A01P3/00A01P1/00
CPCA01H4/008A01H4/001A01H4/002A01N33/12A01N59/08A01N47/28
Inventor 张荣华李松卢曼曼刘俊仙刘红坚刘丽敏周会刘丽辉
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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