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Coaxial optical fiber fluorescence gene detection device and detection method thereof

A fluorescent gene and detection device technology, applied in the field of coaxial optical fiber fluorescent gene detection device, can solve problems such as lack of contrast, low accuracy, and damaged sample molecules

Active Publication Date: 2021-09-07
深圳市核子基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The object of the present invention is to provide a coaxial optical fiber fluorescent gene detection device and its detection method to solve the existing problem: wherein the electrophoretic gel with the same concentration of the sample is manually supplied, and the sample is not subjected to molecular size distinction processing , so it is easy to cause damage to the sample molecules, and the test results are too single, lack of contrast, and the accuracy is not high

Method used

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  • Coaxial optical fiber fluorescence gene detection device and detection method thereof
  • Coaxial optical fiber fluorescence gene detection device and detection method thereof
  • Coaxial optical fiber fluorescence gene detection device and detection method thereof

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Embodiment 1

[0042] This embodiment is used to disclose a coaxial optical fiber fluorescence gene detection device.

[0043] see Figure 1-2 :

[0044] The coaxial optical fiber fluorescent gene detection device includes a molecular centrifugal separation structure 1, which is used to centrifugally separate the molecules of the sample to form molecular layers of different sizes;

[0045] Separation sampling structure 2 is assembled on the outside of molecular centrifugation structure 1, and is used for barrier separation between molecular layers of different sizes;

[0046] The top of the separated sampling structure 2 is connected with a plurality of first capillaries 3, the end of the first capillary 3 away from the separated sampling structure 2 is connected with an automatic electrophoresis gel injection structure 4, and the output end of the automatic electrophoresis gel injection structure 4 is fixedly connected with The soft capillary 5, the end of the soft capillary 5 away from t...

Embodiment 2

[0068] A detection method of a coaxial optical fiber fluorescent gene detection device, which is used in the above embodiments, the steps are as follows:

[0069] Step 1: Place the sample dyed with the fluorescent agent inside the carrier liquid of the sample pool 13, and complete the rapid rotation of the sample inside the sample pool 13 by starting the molecular centrifugal separation structure 1, and fix the inner side of the sample pool 13 in order from the inside to the bottom There are multiple polymer membranes with different densities, and the mixed molecules are centrifuged to make the molecules pass through the polymer membrane. Since the polymer membrane can only pass through smaller molecules, the small molecules are left outside;

[0070] Second step: by the design of the first separation sleeve 31, the second separation sleeve 32 and the third separation sleeve 33, by controlling the second motor 27 to complete the rotation of the first screw rod 28, utilize the s...

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Abstract

The invention discloses a coaxial optical fiber fluorescent gene detection device and a detection method thereof, and relates to the technical field of novel gene detection devices. The device comprises a molecular centrifugal separation structure and a separation sampling structure. Through the cooperative design of the molecular centrifugal separation structure, the separation sampling structure and an automatic electrophoresis gel injection structure, the device is convenient for carrying out centrifugal separation on a detected nucleic acid sample stained with fluorescence in advance, so that nucleic acids with different sizes are separated into multiple layers and are separated to a certain extent; electrophoresis gel with different proportions and concentrations is automatically and cooperatively injected, so that sample damage caused by different molecular sizes and different electrophoresis gel concentrations is avoided, the detection stability and accuracy are further improved, and the device is convenient to better identify and record macromolecules and micromolecules in a separated sample through the design of an optical medium detection structure.

Description

technical field [0001] The invention relates to the technical field of novel gene detection devices, in particular to a coaxial optical fiber fluorescence gene detection device and a detection method thereof. Background technique [0002] With the rapid progress of society, people pay more and more attention to gene detection. The existing gene detection often uses corresponding detection devices. The publication number is a coaxial optical fiber fluorescent gene detection device disclosed by CN211453365U. Although the combination of multiple optical lenses is avoided, the stability of the detection optical path is improved, and it can be used in miniaturized and miniaturized genetic detection devices, but the manual supply of the electrophoretic gel with the same concentration of the sample is used, and the sample is not processed. The differential processing of molecular size can easily lead to damage to the sample molecules, and lead to too single test results, lack of co...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01G01N27/447G01N1/28
CPCG01N21/6428G01N21/01G01N27/44726G01N1/28G01N2021/6439
Inventor 姚杰
Owner 深圳市核子基因科技有限公司
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