Gout diagnosis biomarkers and application thereof

A gout, internal standard technology, applied in the field of gout diagnostic biomarkers, can solve problems such as inability to predict and judge patients

Pending Publication Date: 2021-09-07
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the period of asymptomatic hyperuricemia, it is still impossible to predict and judge whether the patient will develop gout

Method used

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  • Gout diagnosis biomarkers and application thereof
  • Gout diagnosis biomarkers and application thereof
  • Gout diagnosis biomarkers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0097] 1, sample preparation

[0098] Gout and 109 to obtain a blood sample of the patient 102 from the hospital hyperuricemia, metabolites were extracted as follows (all operations were performed on ice):

[0099] ①: 50uL serum samples drawn to the 96-row gun;

[0100] ②: lessons ice discharge gun with 150uL of methanol to the sample;

[0101] ③: After the cover film, the shock 30s, 4 ℃ centrifuge, 4000rpm, 20min;

[0102] ⑤: finished with centrifugation supernatant was added 100uL exhaust suction gun corresponding vial;

[0103] Preparation of quality control

[0104] All samples were taken, after the sample to be thawed on ice, shaking, 10ul of each sample was added to an injection vial, mix, as quality control.

[0105] 2, data collection

[0106] (1) a liquid phase process:

[0107] Instrument: Shimadzu

[0108] Mobile phase: positive and negative ions using the same mobile phase

[0109] A: 25mM NH 4 OH (SIGMA / Fisher) + 25mM NH 4 OAc (SIGMA) + 100% H 2 Of;

[0110] B: ACN;...

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Abstract

The invention relates to gout diagnosis biomarkers and application thereof. The gout diagnosis biomarkers provided by the invention comprise trigonelline, arachidonic acid, betaine, glycocholic acid, pipemidic acid, myristic acid and uracil. The gout diagnosis markers provided by the invention can be detected by adopting a mass spectrum method, and whether a hyperuricemia patient suffers from gout or has a gout suffering risk is determined by using the markers. The invention further comprises a corresponding diagnostic kit.

Description

Technical field [0001] The present invention relates to gout diagnostic biomarkers and their applications. Background technique [0002] Gout patients often have sudden joint pain in the night, inhautive, severe pain, edema, redness and inflammation in the joint site. The most common pathogenesis is the first metataruta, but the pathogenesis is not limited thereto, and it is also common in the joint, knee, elbow, etc. The joints of the disease will eventually be swollen, inflammation, and edema have soft tissue, and the activity is limited. [0003] At present, gout inspection items include: hematuria routine and blood sink, blood uric acid assay, urine test, and joint cavity puncture. Most patients in the acute invasive patient increased the content of serum uric acid. It is generally considered to be determined by uric acid enzyme method, male 420umol / l (7 mg / dl), women 360umol / l (6mg / dl) with diagnostic value. When acute gout arthritis, there may be effirals in the swe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N27/62
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N27/62G01N2030/324
Inventor 沈夏梁宁宁尹慧勇
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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