A bionic "sandwich" structure artificial periosteum and preparation method thereof

A technology of artificial bone and electrospun membrane, which can be used in tissue regeneration, pharmaceutical formulations, conjugated artificial filaments, etc., and can solve the problems of single cell type and inability to simulate periosteal cell structure.

Active Publication Date: 2022-07-26
北京市创伤骨科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method contains a single type of cells and cannot simulate the cellular structure of the different layers of the periosteum.

Method used

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  • A bionic "sandwich" structure artificial periosteum and preparation method thereof
  • A bionic "sandwich" structure artificial periosteum and preparation method thereof
  • A bionic "sandwich" structure artificial periosteum and preparation method thereof

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Embodiment 1

[0053] Example 1. Preparation of biomimetic "sandwich" structure artificial periosteum

[0054] (1) Preparation of fiber layer electrospun membrane

[0055] 1. Pipette 20mL of hexafluoroisopropanol into a 50mL conical flask;

[0056] 2. Weigh 0.85g of PLGA, add it to the above solvent, stir on a magnetic stirrer for 8h to dissolve it;

[0057] 3. Weigh 0.15g of collagen, add it into the above solution system, and continue to stir on a magnetic stirrer for 4h to completely dissolve PLGA and collagen and form a homogeneous solution, wherein the mass content of PLGA is 4.25%, and the mass content of collagen is 0.75%;

[0058] 4. Use a 6mL syringe to suck 6mL of the prepared spinning solution, install the syringe, connecting tube and nozzle in the correct position of the instrument, adjust the spinning parameters to a voltage of 22kV, a receiving distance of 15cm, and an injection speed of 1.0mL / h, the continuous spinning was stopped after 4 hours, the diameter of the fibers ...

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Abstract

The invention discloses a bionic "sandwich" structure artificial periosteum and a preparation method thereof. The biomimetic artificial periosteum comprises a fiber layer electrospinning film and a germinal layer electrospinning film, and at least one of the following cell sheets is laid between the two films to form a sandwich structure: osteoblast precursor cell sheet, mesenchymal stem cell Sheet and vascular endothelial cell sheet; the cell sheet is at least one layer; the fiber layer electrospun membrane is a spinning membrane of degradable polymer materials and natural polymer materials; the germinal layer electrospun membrane is a natural polymer material or natural The spinning film of the composite of polymer material and degradable polymer material. The invention utilizes the nanofiber membrane to be compounded with the cell sheet, which can not only improve the strength of the cell sheet and meet the needs of surgical operations such as suturing, but also utilize the cell shielding effect of the nanofiber membrane to fix the cells between the fiber membranes, effectively Prevent cell proliferation after implantation and effectively improve cell utilization.

Description

technical field [0001] The invention relates to a bionic "sandwich" structure artificial periosteum and a preparation method thereof, in the field of bone defect repair and regeneration research. Background technique [0002] At present, periosteum transplantation is often used in clinical treatment to replace the missing periosteum. Periosteal transplantation techniques include autologous periosteal transplantation, allogeneic periosteal transplantation and artificial periosteal transplantation. Autologous periosteal transplantation has problems such as secondary injury, insufficient periosteum in the donor site, and complications; while allogeneic periosteum has problems such as greater pathogen carrying and the risk of immune rejection. Therefore, it is of great significance to develop a new type of artificial periosteum that can replace the human periosteum. [0003] So far, no "artificial periosteum" product has been used in clinical practice. The substances used to r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/50A61L27/56A61L27/18A61L27/24A61L27/20A61L27/22D01F8/02D01F8/14D01F8/18D04H1/728D04H1/435D04H1/04D04H1/30
CPCA61L27/3821A61L27/3834A61L27/3808A61L27/50A61L27/56A61L27/18A61L27/24A61L27/20A61L27/222D01F8/02D01F8/14D01F8/18D04H1/728D04H1/435D04H1/04D04H1/30A61L2430/02A61L2400/12C08L67/04C08L5/08
Inventor 石锐张静爽
Owner 北京市创伤骨科研究所
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