A kind of cell preservation liquid and its preparation method and application

A technology for preserving liquid and cells, which is applied in the biological field to improve the staining effect, broaden the scope of application, and improve the accuracy and stability.

Active Publication Date: 2022-04-19
广州朗坤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are formaldehyde-free preservation solutions on the market, but there is no preservation solution that can simultaneously meet the four screening methods of thin-layer liquid-based cytology detection, HPV detection, immunocytochemical staining and reduced blue histiocytic staining.

Method used

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  • A kind of cell preservation liquid and its preparation method and application
  • A kind of cell preservation liquid and its preparation method and application
  • A kind of cell preservation liquid and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 A kind of cell preservation solution

[0062] A cell preservation solution, its components and contents are shown in Table 1.

[0063] Components and content of cell preservation solution in Table 1 Example 1

[0064] components content Disodium phosphate 4g Sodium dihydrogen phosphate 8g potassium chloride 5g Sodium chloride 5g calcium chloride 2.5g magnesium chloride 1.25g ethanol 200mL Acetic acid 150mL Trypsin 10g glucose 8g double distilled water Quantitatively make up 1000mL

[0065] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0066] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 600mL double distilled water, stir and dissolve to obtain solution a;

[0067] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chloride to solution a, stir and dissolve to ob...

Embodiment 2

[0071] Embodiment 2 A kind of cell preservation solution

[0072] A cell preservation solution, its components and contents are shown in Table 2.

[0073] Component and content of cell preservation solution in table 2 embodiment 2

[0074] components content Disodium phosphate 5g Sodium dihydrogen phosphate 10g potassium chloride 6g Sodium chloride 6g calcium chloride 3g magnesium chloride 1.5g ethanol 150mL Acetic acid 200mL Trypsin 12g glucose 4g fructose 4g ascorbic acid 2g double distilled water Quantitatively make up 1000mL

[0075] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0076] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 600mL double distilled water, stir and dissolve to obtain solution a;

[0077] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chlori...

Embodiment 3

[0081] Embodiment 3 A kind of cell preservation solution

[0082] A cell preservation solution, its components and contents are shown in Table 3.

[0083] Components and content of cell preservation solution in table 3 embodiment 3

[0084]

[0085]

[0086] The preparation method of the above-mentioned cell preservation solution comprises the following steps:

[0087] (1) Take disodium hydrogen phosphate and sodium dihydrogen phosphate, add 800mL double distilled water, stir and dissolve to obtain solution a;

[0088] (2) Add potassium chloride, sodium chloride, calcium chloride and magnesium chloride to solution a, stir and dissolve to obtain solution b;

[0089] (3) Add trypsin to solution b, stir and dissolve to obtain solution c;

[0090] (4) Add glucose, fructose and ascorbic acid to solution c, stir and dissolve to obtain solution d;

[0091] (5) Add ethanol and acetic acid to the solution d, stir and mix evenly, adjust the pH to 6.5 with NaOH, and distill the...

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Abstract

The invention belongs to the field of biotechnology, and discloses a cell preservation solution, a preparation method and application thereof. The cell preservation solution includes alcohol, acid, buffer salt, ionic strength maintenance agent, antioxidant and proteolytic enzyme; the cell preservation solution can better handle mucus and blood cell samples by adding proteolytic enzyme, and improve thin The staining effect of liquid-based cytology detection; however, proteolytic enzymes will affect the staining effect of reduced blue tissue cells. By adding antioxidants, the effect of proteolytic enzymes on the staining of reduced blue tissue cells is reduced, and at the same time, the immunocytochemical staining is improved. and the accuracy and stability of reducing blue tissue cell staining, and the cell preservation solution has no effect on the accuracy and stability of HPV detection after the cell sample is preserved. That is, the cell preservation solution can simultaneously meet the four screening methods of thin-layer liquid-based cytology detection, HPV detection, immunocytochemical staining and reduced blue histiocyte staining.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cell preservation solution and its preparation method and application. Background technique [0002] Currently, cervical cancer is one of the most common malignant tumors among female cancers. Early diagnosis of cervical cancer and precancerous lesions is the key to improving the cure rate of cervical cancer and the survival rate of patients. Thin-Cytologic Test (TCT) is currently the most widely used clinical method for screening cervical cancer . Thin-layer liquid-based cytology detection is a discipline that diagnoses clinical diseases by observing changes in cell morphology and structure. For decades, thin-layer liquid-based cytology detection has become the main method for large-scale cervical cancer screening and follow-up observation of high-risk groups, which has greatly reduced the incidence of advanced cervical cancer. The smear made by the traditional cyto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12Q1/6851C12Q1/70G01N1/30G01N33/569
CPCA01N1/0226A01N1/0221A01N1/021C12Q1/708C12Q1/6851G01N33/56966G01N1/30G01N2469/10C12Q2531/113C12Q2563/107
Inventor 邹刚军夏婷谭芷晴卢智俊
Owner 广州朗坤生物科技有限公司
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