A method and device for displaying hard tissue pathological slices

A technology for pathological slices and hard tissues, applied in the field of pathological tissue slice preparation, which can solve problems such as poor patch effect, tissue rupture, and incomplete slices, and achieve the effects of short processing time, easy operation, and uniform pressure
CN109187118BActive Publication Date: 2021-01-29XIANGYA HOSPITAL CENT SOUTH UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
XIANGYA HOSPITAL CENT SOUTH UNIV
Publication Date
2021-01-29

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method and a device for spreading a hard tissue pathological section. The method comprises the following steps: 1) fixing a material, dehydrating, clearing, permeating, embedding and trimming with a grinding wheel; 2) uniformly coating an OCT embedding medium on the surface of the hard section and then slicing; 3) putting the sliced hard tissue section into hot water at 45-52 DEG C and spreading the tissue section; 4) using a glass slide coated with chrome alum gelatin for fishing out the hard tissue section; 5) uniformly coating vaseline on a cover glass and placingon the glass slide adhered to the tissue section; 6) placing the glass slide into a pressing device, uniformly applying a pressure, putting the pressing device with the glass slide into an oven at 60DEG C and baking for 2 hours; and 7) taking the glass slide out of the oven, removing the cover glass, putting into xylene for removing vaseline, and naturally airing for later use. The method and thedevice for spreading the hard tissue pathological section disclosed by the invention have the advantages that the operation is convenient, the repeatability is high, the problems of shrinking, shedding, and the like, of the hard tissue section can be effectively avoided, the spreading effect is obviously promoted and the method and the device are suitable for different tissue-engineered bone researches.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of preparation of pathological tissue slices in biology or medicine, in particular to a new method for displaying hard tissue pathological slices. Background technique

[0002] Bone tissue is a kind of hard connective tissue composed of cells, matrix and fibers. The inorganic salt deposited in bone tissue exists in the form of hydroxyapatite, and its main components are calcium phosphate and calcium carbonate. Because bone tissue is hard, brittle, dense, difficult to cut and fragile, it is difficult to slice bone tissue directly in clinical, teaching and scientific research. Decalcified and paraffin-embedded sections of conventional bone tissue often lead to decreased enzyme activity and changes in bone tissue morphology and structure during processing, making it impossible to conduct histological observations, and to study the distribution of calcium and mineralization in tissues. In recent years, the rese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More