Primer pair for identifying capsicum CMV infection resistance and application thereof

A primer pair, pepper technology, applied in the field of agricultural biotechnology engineering, can solve problems such as being unable to meet industrial needs

Pending Publication Date: 2021-10-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many new CMV virus strains can break through the original resistance, so that the existing varieties can no longer meet the needs of the industry

Method used

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  • Primer pair for identifying capsicum CMV infection resistance and application thereof
  • Primer pair for identifying capsicum CMV infection resistance and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] The previous research of the research group found that technologies such as real-time fluorescent quantitative PCR and VIGS have clarified CA02g19570 for qCmr2.1 candidate gene for resistance to CMV. The anti-CMV material PBC688 is the female parent, and the susceptible material G29 is the male parent, and F 1 Hybrid, G29 is the backcross male parent backcross 2 generations to obtain BC 2 group.

[0024] Primer design for molecular markers: According to the presence of a 6-bp insertion in the susceptible material at 277 bp upstream of the start codon ( figure 1 ), take the 150 bp base length on both sides of the InDel site, and a total of 300 bp length is used for primer design. Primer5.0 was used for primer design, the upstream primer design range was 1-150 bp, the downstream primer design range was 156-300 bp, the product size was between 150-250 bp, and the annealing temperature was 52-60 °C.

[0025] The primer sequences for molecular markers are:

[0026] ...

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Abstract

The invention discloses a primer pair for identifying capsicum CMV infection resistance and application of the primer pair. According to the primer pair, a forward primer is as shown in SEQ ID NO.1, and a reverse primer is as shown in SEQ ID NO.2. PCR amplification is performed on a marker by utilizing capsicum genome DNA; if a molecular marker strip of 214 bp exists, the plant is a disease-resistant plant; if a molecular marker strip of 220bp exists, the plant is a diseased plant. By utilizing the marker, an anti-CMV breeding material can be accurately and efficiently screened, offspring single plants carrying resistance genes can be efficiently and accurately screened in hybridization and backcross populations, and compared with conventional anti-CMV breeding, creation of the anti-CMV breeding material and breeding of new varieties can be remarkably accelerated.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology engineering, and relates to a pair of primers for identifying capsicum resistance-sensitivity CMV and an application thereof. Background technique [0002] Cucumber mosaic virus (CMV) is one of the top ten plant viruses in the world, which can infect more than 1200 species of plants in more than 100 families. Liu Yong et al. (2019) survey showed that among the 33 viruses detected in peppers, the detection rate of CMV was as high as 20.29%, surpassing tobacco mosaic virus (detection rate 14.64%) and becoming the most dominant virus that endangered the production of peppers in my country. CMV is a dominant virus that threatens pepper production in my country. Breeding resistant varieties is the most economical and effective method to prevent and control CMV, and it is also the main target of pepper disease-resistant breeding in my country. [0003] Breeders at home and abroad have bred a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 郭广君王述彬潘宝贵刁卫平刘金兵高长洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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