Application of lactobacillus rhamnosus in relieving intestinal inflammation

A technology for Lactobacillus rhamnosus and intestinal inflammation is applied in the application field of Lactobacillus rhamnosus in relieving intestinal inflammation, which can solve the problems of side effects, affecting the quality of life of patients, and being expensive, so as to relieve intestinal inflammation. The effect of verification

Active Publication Date: 2021-10-19
湖北均瑶大健康饮品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used drug treatments are not only expensive but also have side effects, seriously affecting the quality of life of patients. In recent years, lactic acid bacteria have gradually become a research hotspot in the treatment of intestinal diseases due to their safety, easy availability, low cost and no side effects.

Method used

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  • Application of lactobacillus rhamnosus in relieving intestinal inflammation
  • Application of lactobacillus rhamnosus in relieving intestinal inflammation
  • Application of lactobacillus rhamnosus in relieving intestinal inflammation

Examples

Experimental program
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Embodiment 1

[0021] 1. Cultivation of Lactobacillus rhamnosus JL1

[0022] (1) After two generations of L.rhamnosus JL1 activated to the logarithmic growth phase were centrifuged, the bacteria sludge was obtained. After washing with PBS buffer for 3 times, they were resuspended in DMEM culture medium without double antibodies, and the number of colonies was adjusted to 10 8 、10 7 and 10 6 cfu / mL spare. The Caco-2 cells that have reached the polarized state were inoculated in a six-well plate, and the culture medium was DMEM medium without double antibodies, and the culture medium was kept at 37°C, containing 5% CO 2 cultured in an anaerobic incubator, and the medium was changed every other day until the cells reached a polarized state. Take 1mL concentration as 10 8 、10 7 and 10 6 cfu / mL bacterial suspension was added to the above 10 6 Caco-2 cells in 5% CO 2 After co-cultivation in the incubator for 4 h, the culture medium was discarded, and the cells were stimulated with DMEM me...

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Abstract

The invention provides application of lactobacillus rhamnosus in relieving intestinal inflammation, and belongs to the field of probiotic functions. In order to solve the problem that the existing medicines for relieving intestinal inflammation have relatively serious side effects, the invention provides the lactobacillus rhamnosus JL1 which has the function of relieving intestinal inflammation, and the lactobacillus rhamnosus JL1 has the function of relieving intestinal inflammation. Reduction of intestinal inflammation is verified by inhibiting expression of pro-inflammatory genes TNF-alpha, IL-1beta and IL-6 in LPS-induced Caco-2 cells, a clinical state of a mouse and ileum dissection observation.

Description

technical field [0001] The invention belongs to the functional field of probiotics, and in particular relates to the application of Lactobacillus rhamnosus in alleviating intestinal inflammation. Background technique [0002] At present, in terms of relieving intestinal inflammation and intestinal mucosal injury, although conventional drug treatment can reduce the secretion of pro-inflammatory factors to reduce inflammation and damage, long-term use of drugs will bring side effects that are difficult to reverse. Therefore, finding a safe and side-effect-free treatment or a drug to relieve intestinal inflammation has become a research hotspot for scholars from various countries. In recent years, lactic acid bacteria have been widely used in the fields of food and medical treatment due to their natural side effects, low cost and other advantages. Many studies have shown that lactic acid bacteria have a positive effect on the treatment of intestinal inflammation and intestinal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/00A61P29/00A23L33/135
CPCA61K35/747A61P1/00A61P29/00A23L33/135A23V2002/00A23V2400/175A23V2200/3204Y02A50/30
Inventor 姜毓君满朝新张宇杨茉苏悦
Owner 湖北均瑶大健康饮品股份有限公司
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