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Composite nano vaccine for tumor treatment and preparation method thereof

A nano-vaccine and tumor treatment technology, applied in the field of biomedical nano-materials, achieves the effect of uniform size, simple and green method, and high antigen loading rate

Pending Publication Date: 2021-11-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the use of antigens as templates for biomimetic mineralization of metal sulfide materials has not been reported yet.
And after co-incubating nanoparticles with DC, the construction of therapeutic metal sulfide@antigen@DC nanovaccine has not been reported yet.

Method used

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  • Composite nano vaccine for tumor treatment and preparation method thereof
  • Composite nano vaccine for tumor treatment and preparation method thereof
  • Composite nano vaccine for tumor treatment and preparation method thereof

Examples

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preparation example Construction

[0032] The invention provides a method for preparing a composite nano-vaccine for tumor treatment, the method comprising:

[0033] S1. Prepare a metal compound solution and an antigen solution respectively, wherein the concentration of the metal compound solution is 0.05-0.2mol / L, and the concentration of the antigen is 5.71-8.57g / L;

[0034] S2. Slowly add the metal-containing compound solution to the antigen solution, and mix and stir for 0.5-4 hours, wherein the volume ratio of the antigen solution to the metal-containing compound solution is 7:1;

[0035]S3, adding a sulfur-containing compound solution to the reaction system;

[0036] S4, adding a pH regulator to the reaction system to adjust the pH to 10-12, stirring for 10-15 hours to obtain a clarified product;

[0037] S5, washing and filtering the brown clarified product to obtain metal sulfide@antigen nanoparticles;

[0038] S6. Provide immature dendritic cells, and incubate the metal sulfide@antigen nanoparticles ...

Embodiment 1

[0051] Step 1, dissolving chicken ovalbumin (OVA) in double distilled water to form chicken ovalbumin solution, the concentration of chicken ovalbumin is 7.14g / L;

[0052] Step 2, dissolving bismuth nitrate in nitric acid forms bismuth nitrate solution, the concentration of bismuth nitrate solution is 0.05mol / L, and the concentration of nitric acid is 2mol / L;

[0053] Step 3: Slowly add the bismuth nitrate solution to the chicken ovalbumin solution, and mix and stir for 1 hour, wherein the volume ratio of the chicken ovalbumin solution to the bismuth nitrate solution is 7:1, wherein the volume of the bismuth nitrate solution is 5ml, The volume of chicken ovalbumin solution is 35ml;

[0054] Step 4, add 0.5ml sulfur-containing compound solution (1mol / L) to reaction system;

[0055] Step 5, adding a pH regulator to the reaction system to adjust the pH to 12, stirring for 12 hours to obtain a brown-black clarified product;

[0056] Step 6. Use a dialysis bag with a molecular we...

Embodiment 2

[0059] Step 1, dissolving chicken ovalbumin (OVA) in double distilled water to form chicken ovalbumin solution, the concentration of chicken ovalbumin is 5.71g / L;

[0060] Step 2, dissolving bismuth nitrate in nitric acid forms bismuth nitrate solution, the concentration of bismuth nitrate solution is 0.05mol / L, and the concentration of nitric acid is 1mol / L;

[0061] Step 3: Slowly add the bismuth nitrate solution to the chicken ovalbumin solution, and mix and stir for 1 hour, wherein the volume ratio of the chicken ovalbumin solution to the bismuth nitrate solution is 7:1, wherein the volume of the bismuth nitrate solution is 5ml, The volume of chicken ovalbumin solution is 35ml;

[0062] Step 4, add 0.5ml sodium sulfide solution (1mol / L) to reaction system;

[0063] Step 5, adding a pH regulator to the reaction system to adjust the pH to 10, stirring for 12 hours to obtain a brown-black clarified product;

[0064] Step 6, with embodiment one;

[0065] Step seven, same as...

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Abstract

The invention relates to a composite nano vaccine for tumor treatment and a preparation method thereof. The preparation method comprises the following steps that firstly metal sulfide-antigen nano particles are prepared, and then the metal sulfide-antigen nano particles and immature dendritic cells are co-incubated for 2-6 hours to obtain the metal sulfide-antigen-DC composite nano vaccine. According to the composite nano vaccine for tumor treatment and the preparation method thereof, an antigen with high biocompatibility is used as a template and a raw material to synthesize the nano particles, the preparation method is simple and green, and the problem of biosafety when the nano particles are applied to living bodies can be solved; the size of the synthesized metal sulfide nano particles is 10 + / -2.1 nm, and the metal sulfide nano particles are uniform in size, high in stability and high in bioavailability; the synthesized metal sulfide nano particles can be used as a metal adjuvant to stimulate bone marrow cells to induce maturation of immature DC cells, increase antigen presentation and activate downstream T cell related adaptive immune response; the synthesized composite nano vaccine has a high antigen loading rate, and can realize co-delivery of the metal adjuvant and the antigen.

Description

technical field [0001] The invention relates to a composite nano-vaccine for tumor treatment, a preparation method and application thereof, and belongs to the field of biomedical nano-materials. Background technique [0002] In recent years, the incidence of malignant tumors in my country has shown an obvious upward trend. At present, the treatment of malignant tumors is mainly based on radiotherapy and chemotherapy, but there is still a high risk of side effects on normal tissues, which makes the cure rate of cancer still at a very low level. Further development of new therapeutic methods is of great significance. In the past decade, with the rapid advancement of dendritic cell (DC) vaccines and CAR-T cell therapy, immunotherapy, including therapeutic vaccines, has attracted widespread attention in the treatment of tumors. The main components of tumor vaccines include antigens and adjuvants. After the vaccine is injected into the body, it is cross-presented to DC through ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39A61P35/00B82Y5/00B82Y40/00
CPCA61K39/0011A61K39/39A61P35/00B82Y5/00B82Y40/00A61K2039/55505A61K2039/5154
Inventor 汪勇喻欢张乐帅王杨云郭浩翔祖鹤
Owner SUZHOU UNIV
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