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Construction of polyketide synthase PreuA-TEPreu3 and application of polyketide synthase PreuA-TEPreu3 in preparation of orsellinic acid

A technology of polyketide synthase and moss acid is applied in the fields of molecular biology and biochemistry, which can solve the problems of limited application, low yield and high price, and achieve the effect of simple process and high yield.

Active Publication Date: 2021-11-26
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Application Information

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Problems solved by technology

But at present, commercially available orsic acid (CAS: 480-64-8) is produced by chemical synthesis, and the price is about 1,000 yuan / 100mg. The output is low and the price is high, so its application in food, medicine and other fields is restricted. limit

Method used

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  • Construction of polyketide synthase PreuA-TEPreu3 and application of polyketide synthase PreuA-TEPreu3 in preparation of orsellinic acid
  • Construction of polyketide synthase PreuA-TEPreu3 and application of polyketide synthase PreuA-TEPreu3 in preparation of orsellinic acid
  • Construction of polyketide synthase PreuA-TEPreu3 and application of polyketide synthase PreuA-TEPreu3 in preparation of orsellinic acid

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Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with the examples.

[0036] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The materials or equipment used are not indicated by the manufacturer, and they are all conventional products that can be obtained through purchase.

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Abstract

The invention relates to construction of polyketide synthase PreuA-TEPreu3 and application of the polyketide synthase PreuA-TEPreu3 in preparation of orsellinic acid, and belongs to the technical field of molecular biology and biochemistry. According to the invention, polyketide synthase Preu3 and PreuA derived from Preussia isomera are used as materials for the first time, and a novel "non-natural" polyketide synthase PreuA-TEPreu3 is constructed on the basis of a combined biosynthesis technology; the "non-natural" polyketide synthase PreuA-TEPreu3is transferred into saccharomyces cerevisiae to successfully obtain a mutant strain capable of efficiently producing the orsellinic acid; and the prepared orsellinic acid is adopted as an object, the inhibition activity of the orsellinic acid on eight clinical drug-resistant bacteria and seven crop pathogenic fungi is studied, and the strong antagonism (MIC, 12.5 [mu] g / mL) on carbapenem-resistant pseudomonas aeruginosa is found. The method greatly enriches the production sources of the orsellinic acid, and has an important scientific value and application prospect for expanding the derivatization approach of the orsellinic acid and researching and developing a novel antibacterial agent.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and biochemistry, and specifically relates to a novel "non-natural" polyketide synthase PreuA-TE Preu3 Construction and its application in the preparation of orsonic acid. Background technique [0002] Fungal polyketides ( p oly k etide s , PKs) is a large class of important natural product groups with diverse chemical structures and biological activities, and is a high-quality resource library for the discovery of new drug lead compounds. Representative fungal PKs include lovastatin for lowering cholesterol, griseofulvin for fungi, rifamycin for bacteria, mycophenolic acid for immunosuppression, Actin and angiogenesis inhibitor cytochalasin E (cytochalasin E), etc. The key enzyme of PKs biosynthesis - fungal polyketide synthase ( p oly k etide s ynthase, PKS) for iteration (iterative) Type PKS, the domain usually includes: the initiation unit ACP transacylase ( s tarter unit ...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12N15/81C12N1/19C12P7/42C07C51/47C07C65/05A01N37/40A01P3/00A61K31/192A61P31/04C12R1/865
CPCC12N9/1029C12N15/81C12P7/42C07C51/47A01N37/40A61K31/192A61P31/04C07C65/05Y02A50/30
Inventor 刘庆培杨小龙徐瑶张丹
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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