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Tea tree flavonol synthase gene SSR molecular marker primer and application

A technology of flavonol synthase and molecular markers, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Pending Publication Date: 2021-12-10
广西壮族自治区亚热带作物研究所
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Problems solved by technology

[0005] At present, there are no reports on the development of corresponding SSR molecular markers and the application of genetic polymorphisms for the tea tree FLS gene

Method used

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  • Tea tree flavonol synthase gene SSR molecular marker primer and application
  • Tea tree flavonol synthase gene SSR molecular marker primer and application
  • Tea tree flavonol synthase gene SSR molecular marker primer and application

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Embodiment Construction

[0026] Study on SSR Molecular Marker of Key Enzyme Flavonol Synthase (FLS) in Tea Polyphenol Synthesis Pathway of Tea Tree

[0027] (1) Download the mRNA sequence of the flavonol synthase (FLS) gene (GenBank: EF205150), a key enzyme in the tea polyphenol synthesis pathway of tea tree, from NCBI.

[0028] (2) Download the whole genome sequence of the tea tree 'Yunkang No. 10' (download address: http: / / www.plantkingdomgdb.com / tea_tree / dat). Use the SSR search software to find the SSR markers in the whole genome of tea tree, and extract the SSR sequence and its upstream and downstream sequences of specific length (100bp by default). Use primer3 to design primers to obtain primer sequences, SSR repeat motifs and repeat lengths, and pre-amplified fragment lengths. Based on this, 9 pairs of SSR molecular marker primer sequences related to the FLS gene were synthesized.

[0029] (3) Genomic DNA of 72 tea tree germplasm resources (Table 1) was extracted using the kit HYQspin TM CT P...

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Abstract

The invention discloses a tea tree flavonol synthase gene SSR molecular marker (SSR8FLS) and a primer thereof researched and developed by an inventor through a molecular marker technology. Researches show that a method for developing the SSR primer on the whole genome of a tea tree according to specific genes is feasible, and the obtained primer is high in polymorphism and good in repeatability and has good feasibility and pertinence. Therefore, the inventor develops research on genetic polymorphism of tea tree germplasm resources. In conclusion, the research method, the SSR molecular marker and the primer thereof disclosed by the invention can be widely applied to tea tree variety identification, genetic structure and resource diversity analysis, genetic map construction, functional gene positioning and QTL positioning, hybrid seed purity identification and molecular marker-assisted seed selection research, further research on synthesis of phenolic substances of the tea tree and genetic polymorphism of the tea tree is facilitated, and deep development of tea tree resources is promoted.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a tea tree flavonol synthase gene SSR molecular marker primer and its application. Background technique [0002] Tea tree is an important woody economic crop in China, and tea is the oldest and most popular caffeinated beverage in the world. The content of polyphenols in fresh tea leaves is generally between 18% and 36%. They are the main secondary metabolites of tea trees and have a great influence on the formation of tea quality. The composition, content and proportion of polyphenolic compounds in fresh leaves, as well as the degree of transformation and form in the process of tea making, play a decisive role in the formation of tea quality and style, directly affecting the color, aroma and taste of tea. Flavonoids are a class of polyphenolic compounds that widely exist in nature. According to different modifications to the central C ring, they can be div...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/6806C12N15/11
CPCC12Q1/6895C12Q1/686C12Q1/6806C12Q2600/156C12Q2600/13C12Q2525/151
Inventor 彭靖茹
Owner 广西壮族自治区亚热带作物研究所
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