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Tea tree AMP deaminase gene SSR molecular marker primer and application

A technology of molecular markers and deaminases, applied in the field of molecular markers

Pending Publication Date: 2022-05-13
广西壮族自治区亚热带作物研究所
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  • Claims
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Problems solved by technology

The AMP deaminase gene in tea tree was cloned in 2013, but the application research on the corresponding SSR molecular marker and genetic polymorphism of tea tree AMP deaminase gene has not been reported yet

Method used

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  • Tea tree AMP deaminase gene SSR molecular marker primer and application
  • Tea tree AMP deaminase gene SSR molecular marker primer and application
  • Tea tree AMP deaminase gene SSR molecular marker primer and application

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Embodiment Construction

[0028] Study on SSR Molecular Marker of AMP Deaminase Gene in Tea Tree Caffeine Synthesis Pathway

[0029] (1) Download the gene sequence of AMP deaminase gene (GenBank: KC700025) in the caffeine synthesis pathway of tea tree from NCBI.

[0030] (2) Download the whole genome sequence of the tea tree 'Yunkang No. 10' (download address: http: / / www.plantkingdomgdb.com / tea_tree / dat). Use the SSR search software to find the SSR markers in the whole genome of tea tree, and extract the SSR sequence and its upstream and downstream sequences of specific length (100bp by default). Use primer3 to design primers to obtain primer sequences, SSR repeat motifs and repeat lengths, and pre-amplified fragment lengths. Based on this, 9 pairs of SSR molecular marker primer sequences related to AMP deaminase gene were synthesized and prepared.

[0031] (3) The HYQspin TM CT Plant DNA Kit produced by Tiangen Biological Co., Ltd. was used to extract the germplasm resources of 6 tea trees including...

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Abstract

The invention discloses SSR (Simple Sequence Repeat) molecular markers (SSR1-AMP, SSR6-AMP, SSR8-AMP and SSR9-AMP) primers for AMP (Adenosine monophosphate) deaminase genes of tea trees and application of the SSR1 molecular markers. Researches show that the method for developing the SSR primer on the whole genome of the tea tree according to specific genes is feasible, and the obtained primer is high in polymorphism and good in repeatability and has good feasibility and pertinence. Therefore, the inventor develops research on genetic polymorphism of tea tree germplasm resources. In conclusion, the research method, the SSR molecular marker and the primer thereof disclosed by the invention can be widely applied to tea tree variety identification, genetic structure and resource diversity analysis, genetic map construction, functional gene positioning and QTL positioning, seed purity identification and molecular marker-assisted seed selection research, so that further research on tea tree caffeine synthesis and tea tree genetic polymorphism is facilitated; deep development of tea tree resources is promoted.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to primers and applications of tea tree AMP deaminase gene SSR molecular markers. Background technique [0002] Tea is one of the three major beverages in the world. Caffeine is an important component in tea and one of the important substances that affect the quality of tea, accounting for 2% to 5% of the dry weight of tea. In addition to tea, there are at least 60 kinds of plants containing caffeine in the world. Caffeine has many pharmacological effects, so it is loved by people all over the world. At present, caffeine is also widely used in the research and development of drugs to prevent neonatal periodic respiratory arrest, and also plays a very important role in other diseases, such as the treatment of heart disease, myocardial infarction, and the prevention of cancer. [0003] The caffeine in tea tree mainly exists in the leaves, and the young leaves have the high...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6811C12N15/11
CPCC12Q1/6895C12Q1/6811C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 彭靖茹张芬冯春梅黄寿辉甘志勇温立香陈家献袁冬寅檀业维李建强黎新荣
Owner 广西壮族自治区亚热带作物研究所
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