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Tea tree 4-coumaroyl CoA ligase gene SSR molecular marker primers and application

A technology of molecular markers and ligases, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of amplified bands, differences in cluster analysis, different primers and systems, and different principles And other issues

Pending Publication Date: 2021-12-10
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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Problems solved by technology

At present, molecular marker methods mainly include RAPD, RFLP, AFLP, ISSR, SSR, EST-SSR, SRAP, SCoT, etc. These molecular marker methods have different principles, different marker primers and systems, amplified bands, cluster analysis There will also be some differences in

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  • Tea tree 4-coumaroyl CoA ligase gene SSR molecular marker primers and application
  • Tea tree 4-coumaroyl CoA ligase gene SSR molecular marker primers and application
  • Tea tree 4-coumaroyl CoA ligase gene SSR molecular marker primers and application

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Embodiment Construction

[0027] Molecular markers of key enzyme 4-coumaroyl-CoA ligase (4CL) in the synthetic pathway of tea polyphenols in tea tree

[0028] (1) Download the mRNA sequence of the key enzyme 4-coumaroyl-CoA ligase (4CL) gene (GenBank: DQ194356) in the tea polyphenol synthesis pathway of tea tree from NCBI.

[0029] (2) Download the whole genome sequence of the tea tree 'Yunkang No. 10' (download address: http: / / www.plantkingdomgdb.com / tea_tree / dat). Use the SSR search software to find the SSR markers in the whole genome of tea tree, and extract the SSR sequence and its upstream and downstream sequences of specific length (100bp by default). Use primer3 to design primers to obtain primer sequences, SSR repeat motifs and repeat lengths, and pre-amplified fragment lengths. Based on this, 17 pairs of SSR molecular marker primer sequences related to 4CL gene were synthesized.

[0030] (3) Genomic DNA of 72 tea tree germplasm resources (Table 1) was extracted using the kit HYQspin TM CT Pl...

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Abstract

The invention discloses tea tree 4-coumaroyl CoA ligase gene SSR molecular markers (SSR14 4CL and SSR15 4CL) and primers thereof which are researched and developed by an inventor by utilizing a molecular marker technology. Researches show that a method for developing the SSR primers on the whole genome of a tea tree according to specific genes is feasible, and the obtained primers are high in polymorphism and good in repeatability and have good feasibility and pertinence. Therefore, the inventor develops research on genetic polymorphism of tea tree germplasm resources. In conclusion, the research method, the SSR molecular markers and the primers thereof disclosed by the invention can be widely applied to tea tree variety identification, genetic structure and resource diversity analysis, genetic map construction, functional gene positioning, QTL positioning and molecular marker-assisted seed selection research, so that further research on tea tree phenolic substance synthesis and tea tree genetic polymorphism is facilitated, and deep development of tea tree resources is promoted.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to SSR molecular marker primers and applications of tea tree 4-coumaroyl CoA ligase gene. Background technique [0002] Tea tree is an important woody economic crop in China, and tea is the oldest and most popular caffeinated beverage in the world. The content of polyphenols in fresh tea leaves is generally between 18% and 36%. They are the main secondary metabolites of tea trees and have a great influence on the formation of tea quality. The composition, content and proportion of polyphenolic compounds in fresh leaves, as well as the degree of transformation and form in the process of tea making, play a decisive role in the formation of tea quality and style, directly affecting the color, aroma and taste of tea. [0003] In the synthetic pathway of tea tree phenols, 4-coumaric acid acetyl ligase (4-coumaroyl CoA ligase, 4CL) is one of the key enzymes, which mainly cataly...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 彭靖茹温立香檀业维黄寿辉张芬甘志勇冯春梅陈家献袁冬寅赵媛
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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