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Autologous platelet factor gel as well as preparation method and application thereof

A platelet factor and gel technology, used in prosthesis, transportation and packaging, anticoagulation treatment, etc., can solve the problems of complex process, unsatisfactory medical aesthetic effect, poor filling effect, etc. Slow absorption effect

Active Publication Date: 2021-12-24
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of PRP by secondary centrifugation requires adding an anticoagulant (commonly used sodium citrate), which is simple to operate, high in extraction rate, wide in clinical application, and rich in application cases (osteoarthritis, etc.); however, exogenous thrombin is required for activation. Calcium ions, and the effect of medical aesthetics is not ideal, and the filling effect is poor
[0010] By continuously adjusting the rotational speed during the centrifugation process, a better gel can be obtained, the plasticity of the gel can be increased, and the membrane can be formed, etc., which can be used for osteoinductive growth, but the process is more complicated, and a specific centrifugation program needs to be established. Similarly, immediately after collection preparation, operation immediately after preparation

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  • Autologous platelet factor gel as well as preparation method and application thereof
  • Autologous platelet factor gel as well as preparation method and application thereof
  • Autologous platelet factor gel as well as preparation method and application thereof

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preparation example Construction

[0041] The invention provides a preparation method of autologous platelet factor gel, which has flexible and unlimited time, one-time blood collection and multiple operations, and the obtained autologous platelet factor gel has excellent immediate effect, slow absorption, long duration of effect, and safety , Convenience, storability and other advantages.

[0042] next combine Figure 1 to Figure 2 The technical solution of the present invention will be further described with specific examples.

[0043] In embodiment 1 to embodiment 3, the specific method of collecting autologous whole blood is as follows:

[0044] Take 10-50ml whole blood from a healthy adult from the antecubital vein, store the blood in an anticoagulant tube containing EDTA, and complete the cryopreservation process within 2 hours.

[0045] The first blood sample was centrifuged at 600×g for 8 minutes. After the centrifugation was completed, the upper plasma was drawn into the centrifuge tube;

[0046] Th...

Embodiment 1

[0050] The invention provides a preparation method of autologous platelet factor gel, comprising the following steps:

[0051] Step S1, collect autologous whole blood, first centrifuge at 600×g for 8 minutes, draw the upper plasma into a centrifuge tube after centrifugation; the plasma contains platelet-poor plasma (PPP) and platelet-rich plasma (PRP);

[0052] Step S2, centrifuge the obtained plasma at a speed of 1500×g for 8 minutes, take the upper three-quarters of the centrifugate as the upper layer, take the lower one-quarter of the centrifugation as the lower layer, and place the upper layer PPP in- Store at 20°C for later use, and store the lower liquid PRP at -80°C for later use;

[0053] Step S3. Physically modify the PPP in the supernatant liquid stored in step S2 to form a gel. The modification method is: after freezing and thawing at -20°C, heat in a water bath at 75°C for 15 minutes;

[0054] Step S4, the lower layer liquid PRP preserved in step S2 undergoes phys...

Embodiment 2

[0061] The invention provides a preparation method of autologous platelet factor gel, comprising the following steps:

[0062] Step S1, collect autologous whole blood, first centrifuge at 600×g for 8 minutes, draw the upper plasma into a centrifuge tube after centrifugation; the plasma contains platelet-poor plasma (PPP) and platelet-rich plasma (PRP);

[0063] Step S2, centrifuging the obtained plasma at a speed of 1500×g for 8 minutes, taking four-fifths of the upper layer of the centrifugation liquid as the upper layer liquid, taking one-fifth of the lower layer of the centrifugation liquid as the lower layer liquid, and placing the upper layer PPP in- Store at 20°C for later use, and store the lower liquid PRP at -80°C for later use;

[0064] Step S3. Physically modify the PPP of the supernatant liquid stored in step S2 to form a gel. The modification method is: freeze-thaw at -20°C, and heat in a water bath at 75°C for 15 minutes;

[0065] Step S4, the lower layer liquid...

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Abstract

The invention provides an autologous platelet factor gel as well as a preparation method and application thereof. The preparation method comprises the following steps that S1, autologous whole blood is collected, and centrifugal separation is performed to obtain plasma; the plasma comprises platelet-poor plasma (PPP) and platelet-rich plasma (PRP); S2, the obtained plasma is centrifuged to obtain centrifugate, and supernatant PPP and subnatant PRP are stored for later use respectively; S3, physical modification is performed on the obtained supernatant to form the gel; S4, physical cracking is performed on the obtained subnatant to release factors; and S5, the gel obtained in the step S3 and the subnatant obtained after physical cracking in the step S4 are mixed uniformly to obtain the autologous platelet factor gel. According to the preparation method of the autologous platelet factor gel, the time is flexible, no limitation is exist, one-time blood sampling and a plurality of operations are achieved, and according to the obtained autologous platelet factor gel, the instant effect is excellent, the absorption is slow, the effect duration is long, and the obtained autologous platelet factor gel has the advantages of safety, convenience, storability and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an autologous platelet factor gel and its preparation method and application. Background technique [0002] Autologous platelet-rich plasma (Platelet-rich plasma, PRP) is the platelet concentrate produced by autologous blood. A large number of clinical studies have reported that PRP contains a large number of various platelet-derived cell growth factors, which can promote the repair of various tissues. A variety of biological products are currently derived from PRP: [0003] 1. Platelet-rich plasma (PRP): [0004] Platelet-rich plasma (platelet-rich plasma, PRP) is the plasma containing high volume fraction of platelets obtained by centrifuging the whole blood of human or animal itself. The preparation of PRP by secondary centrifugation requires adding an anticoagulant (commonly used sodium citrate), which is simple to operate, high in extraction rate, wide in cli...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/52A61L33/00A61L33/04
CPCA61L27/3616A61L27/52A61L27/3691A61L27/3834A61L33/0041A61L33/04
Inventor 王翔王洁海泉
Owner CHENGDU QINGKE BIOTECH
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