Plant cell hybridization technology

Abstract

The invention discloses a plant cell hybridization technology. The plant cell hybridization technology comprises the following steps: (1) selecting equipment and raw materials, specifically, preparing a culture dish, a constant-temperature incubator, a crusher, a shake flask machine, an ultra-clean workbench, a sterile room, an experimental field, absorbent paper and an ultraviolet lamp, and preparing a culture medium, an explant, hydrogen peroxide, sterile water, normal saline, distilled water, cellulase, sugar and agar; and (2) conducting receptor culture, specifically, according to the selected plant species, mixing appropriate culture nutritional ingredients into agar to obtain a culture medium for culturing the plant. According to the cell hybridization technology of the plant, receptor calluses are independently cultured, so that genes with excellent characters enter receptor cells through the cell hybridization technology, integration and transmission of the excellent characters are achieved, the hybridization culture speed is higher, the culture time is shortened, plants with various excellent characters are obtained more quickly, and therefore, the plant culture is more efficient.

Description

technical field [0001] The invention relates to the technical field of plant hybridization, in particular to a plant cell hybridization technology. Background technique [0002] Hybridization is a way to obtain two different excellent traits by pairing the same plant with different traits. Plant hybridization can artificially intervene in plant growth traits, so that a single plant has multiple excellent traits. In order to obtain excellent plant types, and the current development of biotechnology, using the totipotency of plant cells, through scientific cultivation methods, allows the hybridization to enter the cell level, and through cell differentiation and cultivation, it greatly shortens the time required for plant hybridization. Time, through the tissue culture of plants, can obtain the same and stable hereditary plants as the plants, and this feature also provides the basis for the excellent traits of the plants, but the existing hybridization technology still has the...

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of plant cell hybridization technology, in order to solve above-mentioned existing existing plant that proposes and there is bigger interval difference and interval resistance, wherein the interval difference between plants makes plant It is difficult to produce stable genetic offspring during traditional hybridization, and the interval resistance between plants makes it impossible to introduce target genes into recipient cells using cell hybridization technology, resulting in the inability to obtain cells with a variety of good shape genes, and also It is impossible to obtain excellent plants through tissue culture. In the process of cell hybridization of existing plants, due to the effect of interval resistance, when the plants undergo subsequent differentiation culture, they are prone to the problem of interval inadaptability, resulting in plants that cannot be normal. The occurrence of problems such as low survival rate of plant cultivation and transplantation makes plant cultivation require more time, and it is impossible to achieve short-term cultivation. The existing plants are not suitable for the interval in the process of cell hybridization, resulting in poor traits. It can be well inherited to the next generation, and it is impossible to obtain excellent and stable genetic plants, resulting in the interruption of the transmission of excellent traits, which requires continuous cell hybridization, which makes the time-saving and labor-saving advantages of cell hybridization technology lost.