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Widmannella terrestris with high yield of EPA grease and application of widmannella terrestris

A technology of welchterella and welchterella algae, which is applied in the field of welchtonii algae strains, can solve problems such as insufficient EPA potential and decreased EPA content, and achieve the effects of source of excellent algae species, strong stress resistance, and reduction of production costs

Pending Publication Date: 2022-01-21
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

However, as the salinity of seawater increases to >15%, the content of EPA decreases rapidly, that is, the potential for producing EPA is insufficient

Method used

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  • Widmannella terrestris with high yield of EPA grease and application of widmannella terrestris
  • Widmannella terrestris with high yield of EPA grease and application of widmannella terrestris
  • Widmannella terrestris with high yield of EPA grease and application of widmannella terrestris

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Embodiment Construction

[0039] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The examples are only used to explain the present invention, not to limit the protection scope of the present invention.

[0040] The present invention isolates a strain of Widleri algae from the desert biological soil crust, which not only has strong stress resistance, but also can meet the growth demand by adding some simple nutrients, and can produce such as EPA with relatively low production cost and has high economical performance. Fatty acid of benefit; therefore, the isolated Wischerella algae strain of the present invention is an oleaginous microalgae with great application potential.

[0041] (1) Collection, cultivation and isolation of desert wilsonii

[0042] Samples of biological soil crusts were collected from the desert area of ​​Ningxia and stored in sterile sealed bags. After being sent to the laboratory, rinse with sterile dei...

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Abstract

The invention discloses widmannella terrestris with high yield of EPA grease and an application of the widmannella terrestris. Algal strain WL1 separated from desert organism soil crust is subjected to 18S rDNA sequence analysis and microscopic morphology identification to determine that the algal strain WL1 belongs to Widmannella. According to the algal strain, in a batch culture mode of the algal strain, neutral fat accumulated in algal cells accounts for more than 80% of total fat, fatty acid mainly comprises palmitoleic acid, oleic acid and linolenic acid and can account for more than 70% of total fat fatty acid, the algal strain is suitable for production of biodiesel, contains unsaturated fatty acid eicosapentaenoic acid required by a human body, and the content of the fatty acid accounts for more than 10% of the total fatty acid.

Description

technical field [0001] The invention relates to a wilsoni algae strain capable of accumulating a large amount of high-EPA oil in algae cells and its application, in particular to the isolation, identification and cultivation of a wilsoni algae strain. Background technique [0002] Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid, which is an important fatty acid that cannot be synthesized by the human body but is indispensable. Increasing the intake and absorption of EPA on the basis of daily diet has obvious effects on the prevention and treatment of arteriosclerosis, cerebral thrombosis and hypertension. The development and utilization of EPA has become one of the hotspots in the field of health care products and medicine. At present, the commercial source of EPA is mainly deep-sea fish oil, but the production cost of deep-sea fish oil is high, and the output cannot meet the market demand. With the expanding application and increasing demand of EPA, it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02C12P7/6427C12P7/64C12P7/649C12R1/89
CPCC12N1/12C12N1/02C12P7/6427C12P7/64C12P7/649Y02E50/10
Inventor 高翔佘洋景欣
Owner SHAANXI UNIV OF SCI & TECH
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