A microfluidic chip and method for realizing fast and accurate cell sorting at low flow rate

Abstract

The invention discloses a microfluidic chip and a method for realizing rapid and precise cell sorting under low flow rate, comprising a microfluidic chip flow channel layer, the microfluidic chip flow channel layer comprising a sample flow double-split inlet flow channel, Sheath flow inlet flow channel, extrusion flow channel, contraction expansion array flow channel, sorting flow channel and sudden expansion sorting flow channel. Viscoelastic solution is used for viscoelastic sheath flow and viscoelastic sample flow. Different degrees of lateral migration are carried out in the channel according to its size, which realizes rapid sorting in a simple channel structure, and at the same time, it is carried out at a low flow rate, which has a certain protective effect on vulnerable cells.

Description

technical field [0001] The invention relates to the field of biological particle manipulation based on a microfluidic chip, in particular to an integrated viscoelastic flow channel wall surface and flow channel center double-balanced position focusing technology and a shrink-expanded array flow channel sorting technology to realize the size-based sorting of cells. Microfluidic chip and method. Background technique [0002] At present, the detection of rare cells such as circulating tumor cells (CTCs) has been widely used in the early diagnosis, prognosis evaluation, efficacy detection and personalized treatment of patients with malignant tumors, so the rare cell detection equipment with simple operation and high accuracy has become a clinical application Research hotspots. [0003] Microfluidic chips have great development potential and broad application prospects in the detection and sorting of rare cells due to their advantages of small sample volume, low cost, simple ope...

AI Technical Summary

Problems solved by technology

In order to achieve effective cell sorting, the common Newtonian fluid inertial microfluidic technology is usually carried out at a higher flow rate, and the sorting efficiency is higher, but the manipulation accuracy for small-sized cells is limited, and it is not friendly to fragile cells

In the inertial straight channel, the flow rate of the viscoelastic fluid is low, and the efficiency of cell manipulation is limited to a certain extent, but the particle has a higher discrimination accuracy.

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