Methods, apparatus, equipment, media and program products for single-cell sequencing
A single-cell sequencing and nanopore sequencing technology, applied in the field of single-cell sequencing, can solve problems such as differences in genetic information and loss of low-abundance information
Active Publication Date: 2022-07-15
BIOMAP (BEIJING) INTELLIGENCE TECH LTD
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Due to cell heterogeneity, the genetic information of cells of the same phenotype may be significantly different, and many low-abundance information will be lost in the overall representation
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[0024] In the present disclosure, unless otherwise specified, the use of the terms "first", "second", etc. to describe various elements is not intended to limit the positional relationship, timing relationship or importance relationship of these elements, and such terms are only used for Distinguish one element from another. In some examples, the first element and the second element may refer to the same instance of the element, while in some cases they may refer to different instances based on the context of the description.
[0025] The terminology used in the description of the various described examples in this disclosure is for the purpose of describing particular examples only and is not intended to be limiting. Unless the context clearly dictates otherwise, if the number of an element is not expressly limited, the element may be one or more. As used herein, the term "plurality" means two or more, and the term "based on" should be construed as "based at least in part on...
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Provided is a method for single-cell sequencing, comprising: obtaining multiple nucleotide sequences in a sequencing library and nanopore sequencing signals corresponding to the multiple nucleotide sequences; performing a first clustering of the nucleotide sequences to obtain a first plurality of clusters, the first plurality of clusters including the largest cluster having the largest cluster size; signals based on the corresponding nanopore sequencing signals of the plurality of nucleotide sequences The length mean value and the nanopore sequencing signal corresponding to each nucleotide sequence in the largest cluster are determined, and the merge threshold is determined; based on the second similarity threshold, the first clustering is performed on the plurality of nucleotide sequences to obtain a second plurality of nucleotide sequences. Clustering, the first similarity threshold is greater than the second similarity threshold; and clustering optimization is performed on the second plurality of clusterings based on the merging threshold to obtain the third plurality of clusterings.
Description
technical field [0001] The present disclosure relates to the technical field of single-cell sequencing, and in particular, to methods, apparatuses, electronic devices, computer-readable storage media, and computer program products for single-cell sequencing. Background technique [0002] With the continuous development of second- and third-generation sequencing technologies, great changes have been brought about in the field of biology. Initially, researchers took enough DNA samples from large numbers of cells and sequenced them. Such sequencing results are the "average" results of these DNA samples. Due to cellular heterogeneity, the genetic information of cells of the same phenotype may differ significantly, and much low-abundance information is lost in the overall characterization. In order to make up for the limitations of traditional high-throughput sequencing, single-cell sequencing technology came into being. [0003] Single-cell sequencing technology refers to a n...
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IPC IPC(8): G16B20/30G16B30/00G06K9/62
CPCG16B20/30G16B30/00G06F18/23213G06F18/23
Inventor 韩仁敏高欣祁俊海
Owner BIOMAP (BEIJING) INTELLIGENCE TECH LTD