Preparation method and application of umbilical cord mesenchymal stem cell nutrient solution

A technology of mesenchymal stem cells and nutrient solution, applied in the field of biological cell preparation, can solve the problem of less utilization of mesenchymal stem cells, and achieve the effect of promoting repair

Pending Publication Date: 2022-03-15
孟明耀 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the use of mesenchymal stem cells is still relatively small. The applicant combines long-term clinical practice research to improve its utilization and conduct research on application methods in the fields of medicine and beauty.

Method used

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  • Preparation method and application of umbilical cord mesenchymal stem cell nutrient solution
  • Preparation method and application of umbilical cord mesenchymal stem cell nutrient solution
  • Preparation method and application of umbilical cord mesenchymal stem cell nutrient solution

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Embodiment 1

[0043] A method for preparing umbilical cord mesenchymal stem cell nutrient solution, comprising the following steps:

[0044] S1. Isolation of stem cells: Isolation of mesenchymal stem cells from the corresponding tissue of human or mammalian umbilical cord tissue;

[0045] S2. Stem cell culture: add 10% fetal calf serum to the α-MEM medium of the mesenchymal stem cells in the above steps, 5% CO2, culture at 37°C for 3 days, and then replace the complete culture medium at intervals of 3 days Once, subculture to obtain the desired stem cells;

[0046] S3. Preparation of cell nutrient solution: After culturing mesenchymal stem cells for 2 to 3 generations, add 3 mL of 0.25% trypsin, then add 10 mL of α-MEM medium containing fetal bovine serum to stop digestion, collect the cell suspension, and then Centrifuge at normal room temperature for 5 minutes to obtain the supernatant, which is the cell nutrient solution.

[0047] Wherein the specific method of the S1 step is:

[0048...

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Abstract

The invention relates to the technical field of biological cell preparation, in particular to a preparation method and application of an umbilical cord mesenchymal stem cell nutrient solution. The invention aims to provide a cell nutrient solution which can well repair skin cells and has a good beautifying effect and an application method of the cell nutrient solution. According to the technical scheme, the method comprises the following steps: separating mesenchymal stem cells, culturing the stem cells, and preparing a cell nutrient solution; the mesenchymal stem cell nutrient solution obtained by the invention comprises high-concentration human umbilical cord blood mesenchymal stem cells; a supernatant of the kit contains vascular endothelial cell growth factors, basic fibroblast growth factors, epidermal growth factors, IL-6 and IL-7, megakaryocyte colony stimulating factors, tumor necrosis factors, interferon and other factors, deoxyribonucleic acid and ribonucleic acid, monocyte chemotactic proteins, intercellular adhesion molecules, active polypeptides, immune proteins IgG, IgA, IgM, IgD, IgE and the like. The repair of tissue defects can be well promoted.

Description

technical field [0001] The invention relates to the technical field of biological cell preparation, in particular to a method for preparing umbilical cord mesenchymal stem cell nutrient solution and its application. Background technique [0002] Mesenchymal stem cells refer to a class of stem cell subgroups with the potential to differentiate into three germ layers. Under certain circumstances, they can theoretically be induced to differentiate into any tissue cells in the human body. Bone marrow, fat, umbilical cord and placenta, etc., are more and more concerned about their clinical application value because of their strong proliferation ability, strong differentiation ability, low immunogenicity and immune regulation function. At the same time, stem cells contain a large number of biologically active substances. Stem cell supernatants contain vascular endothelial growth factor, basic fibroblast growth factor, epidermal growth factor, IL-6 and IL-7, megakaryocyte colony st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K8/99A61K35/28A61P17/02A61Q19/00
CPCC12N5/0668A61K35/28A61P17/02A61K8/99A61Q19/00C12N2509/00
Inventor 孟明耀侯宗柳刘师节李琳高慧赵旖旖王晓丹
Owner 孟明耀
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