Preparation method and application of umbilical cord mesenchymal stem cell nutrient solution
A technology of mesenchymal stem cells and nutrient solution, applied in the field of biological cell preparation, can solve the problem of less utilization of mesenchymal stem cells, and achieve the effect of promoting repair
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[0043] A method for preparing umbilical cord mesenchymal stem cell nutrient solution, comprising the following steps:
[0044] S1. Isolation of stem cells: Isolation of mesenchymal stem cells from the corresponding tissue of human or mammalian umbilical cord tissue;
[0045] S2. Stem cell culture: add 10% fetal calf serum to the α-MEM medium of the mesenchymal stem cells in the above steps, 5% CO2, culture at 37°C for 3 days, and then replace the complete culture medium at intervals of 3 days Once, subculture to obtain the desired stem cells;
[0046] S3. Preparation of cell nutrient solution: After culturing mesenchymal stem cells for 2 to 3 generations, add 3 mL of 0.25% trypsin, then add 10 mL of α-MEM medium containing fetal bovine serum to stop digestion, collect the cell suspension, and then Centrifuge at normal room temperature for 5 minutes to obtain the supernatant, which is the cell nutrient solution.
[0047] Wherein the specific method of the S1 step is:
[0048...
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