Botanical bactericidal composition for preventing and treating rice sheath blight disease, preparation and application of botanical bactericidal composition

A technology of rice sheath blight and plant origin, applied in the field of pesticides, can solve the problems of Melaleuca alternifolia and borneol activity that have not been reported yet, and achieve the effect of improving crop immune system ability, rich natural resources, and low equipment requirements

Active Publication Date: 2022-03-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of Melaleuca alternifolia and Borneol on anti-phytopathogenic bacteria has not been reported yet

Method used

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  • Botanical bactericidal composition for preventing and treating rice sheath blight disease, preparation and application of botanical bactericidal composition
  • Botanical bactericidal composition for preventing and treating rice sheath blight disease, preparation and application of botanical bactericidal composition
  • Botanical bactericidal composition for preventing and treating rice sheath blight disease, preparation and application of botanical bactericidal composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of Melaleuca alternifolia extract

[0046] Preparation of Melaleuca alternifolia extract: The fresh stems and leaves of Melaleuca alternifolia collected in Dongba Village, Jiantou Town, Dongyuan County, Heyuan City, Guangdong Province were cut into pieces, dried in a blower drying oven at 52°C, and crushed. 60-mesh sieve to obtain Melaleuca alternifolia powder; add Melaleuca alternifolia powder and 75% ethanol according to the ratio of material to liquid (g:mL) 1:10, and ultrasonically extract at 50°C for 6 hours to obtain an extract; The extract is concentrated in vacuum to an extract with a water content of 3% to 5%. The extract is dissolved in water and extracted with petroleum ether, ethyl acetate, and n-butanol in sequence to obtain an extract; the extract is concentrated to obtain n-butanol extract part of the extract, and the extract part extracted with n-butanol is Melaleuca alternifolia extract.

Embodiment 2

[0047]Embodiment 2: Determination of in vitro antibacterial activity of Melaleuca alternifolia extract and Borneolum to Rhizoctonia solani

[0048] (1) Experimental object: Rhizoctonia solani

[0049] (2) Experimental method: According to the agricultural industry standard of the People's Republic of China (NY / T 1156.2-2006), the mycelium growth rate method was used for determination.

[0050] (3) Drugs to be tested: Melaleuca alternifolia extract prepared in Example 1, borneol (purity 80%).

[0051] (4) Preparation of medicament mother liquor: the Melaleuca alternifolia extract and borneol prepared in Example 1 were dissolved in dimethyl sulfoxide respectively, and prepared into mother liquors of different concentrations for later use.

[0052] (5) Drug treatment: under aseptic conditions, the pre-melted PDA medium is added in a sterile Erlenmeyer flask, then the mother liquors of different concentrations prepared in step (4) are drawn and added to the above-mentioned PDA me...

Embodiment 3

[0060] Embodiment 3: the combined toxicity assay method of Melaleuca alternifolia extract and Borneol

[0061] According to the toxicity test results of Example 2, let the lethal intermediate concentration of Melaleuca alternifolia extract be a, and the lethal intermediate concentration of borneol be b. When using the co-toxicity factor method to evaluate the synergistic effect of the Melaleuca alternifolia extract prepared in Example 1 and borneol, select 5 proportions, and these 5 proportions are the sixth grade of the additive action line in the isobolism method. Points can be expressed as a / 6b, b / 2b, a / b, 2a / b, 5a / b. The concentrations of these five proportioning mixtures are (a+5b) / 6, (a+2b) / 3, (a+b) / 2, (2a+b) / 3, (5a+b) / 6.

[0062] The specific preparation method is: dissolve and dilute the Melaleuca alternifolia extract with dimethyl sulfoxide to obtain a 780.23 mg / L Melaleuca alternifolia extract solution (that is, to dilute the Melaleuca alternifolia extract so that i...

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Abstract

The invention discloses a botanical bactericidal composition for preventing and treating rice sheath blight disease, a preparation and application of the botanical bactericidal composition and the preparation, and belongs to the technical field of pesticides. The plant source composition for preventing and treating the rice sheath blight disease comprises a melaleuca alternifolia extract and borneol. The melaleuca alternifolia extract and borneol in the botanical bactericidal composition for preventing and treating the rice sheath blight disease have a synergistic effect when being compounded in a mass ratio of 49: 1, the effect of preventing and treating botanical diseases caused by rhizoctonia solani is obvious, drug resistance is not easy to generate, meanwhile, the capability of a crop immune system can be improved, and the activity of the crop immune system can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a botanical fungicidal composition, preparation and application thereof for preventing and treating rice sheath blight. Background technique [0002] Phytopathogens are agricultural microorganisms that can induce a variety of plant diseases, causing losses of more than 200 billion US dollars in a few decades, and posing a great threat to global food production. For example, rice sheath blight, caused by Rhizoctonia solani, is considered one of the most devastating rice diseases worldwide. Rice is the most important cereal crop in the world, and it is also the most important economic crop and food crop in my country. The prevalence of rice sheath blight in China is as high as 15 million to 20 million hectares, causing an annual output loss of about 6 million tons. At present, chemical pesticide control is the key to reduce the incidence of rice sheath blight. Due to the long-t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/28A01N31/06A01N25/04A01P3/00
CPCA01N65/00A01N65/28A01N31/06A01N25/04
Inventor 汤日元熊兰图
Owner SOUTH CHINA AGRI UNIV
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