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A kind of microenvironment NK cell immune regulation delivery system and preparation method and application thereof

A technology of NK cells and immune regulation, applied in the fields of chemistry and biomedical engineering, can solve the problems of lack of non-specific drug absorption, and achieve the effects of reducing drug leakage, stabilizing structure, reducing distribution and release

Active Publication Date: 2022-08-09
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In summary, there is currently a lack of nanocarrier systems that can effectively avoid maternal and fetal non-specific drug absorption, thereby achieving selective drug delivery and functional regulation of natural killer cells in the placenta

Method used

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  • A kind of microenvironment NK cell immune regulation delivery system and preparation method and application thereof
  • A kind of microenvironment NK cell immune regulation delivery system and preparation method and application thereof
  • A kind of microenvironment NK cell immune regulation delivery system and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] S1. Synthesis of Polyacetimide Grafted Polyethylene Glycol (PEG-PEI)

[0050] A two-step method was used to synthesize polyethyleneimine-grafted polyethylene glycol (PEG-PEI). First, the terminal hydroxyl group of monomethyl ether polyglycol was activated with carbonyldiimidazole, and then reacted with the amino group of polyethyleneimine to form PEG. -PEI. The specific operation is as follows: Weigh monomethyl ether ethylene glycol (8.0 g, Mn=2 kDa) into a reaction flask, vacuum dry at 80° C. for 6 h, and add THF (60 mL) to dissolve it under argon atmosphere. Weigh carbonyldiimidazole (CDI, 6.4 g) into another reaction flask, and then slowly drop the THF dissolved with mPEG-OH into the CDI flask with a constant pressure dropping funnel, and stir the reaction at room temperature overnight. Distilled water (0.648 mL) was added to inactivate excess CDI, and stirring was continued for 30 min. The solution was precipitated into a large amount of cold ether, filtered and d...

Embodiment 2-4， comparative example 1-6

[0071] Compared with Example 1, changing the feeding amount of polymer, drug and SPIO in step S3 or omitting a certain step in steps S3, S4, S5, S6, S7 can prepare Example 2-4 or Comparative Example 1-6. , see Table 1 below for details:

[0072] Table 1: Examples and Comparative Examples

[0073]

[0074]

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Abstract

The invention discloses a microenvironmental NK cell immune regulation and delivery system and a preparation method and application thereof, including: the shell is an enzyme substrate polypeptide-PEG modified with targeted disintegration under the action of an enzyme highly expressed in contact with placental interstitial fluid A lipid bimolecular membrane; the inner core is a drug carrier modified with a marker antibody that is specifically and highly expressed on the surface of placental natural killer cells; the drug carrier is loaded with superparamagnetic ferric oxide SPIO nanoparticles to regulate the function of placental natural killer cells. Molecular drugs, therapeutic genes, or combinations thereof. The microenvironmental NK cell immune regulation delivery system of the present invention can effectively avoid the absorption of non-specific drugs by the mother and the fetus, thereby realizing the delivery of natural killer cell-specific drugs in the placenta and the regulation of immune function.

Description

technical field [0001] The invention relates to the fields of chemistry and biomedical engineering, in particular to a microenvironmental NK cell immune regulation and delivery system and a preparation method and application thereof. Background technique [0002] Gestational diabetes mellitus (GDM) refers to abnormal glucose metabolism that first occurs or is discovered during pregnancy. With the improvement of living standards, changes in dietary structure and increasing maternal age, the incidence of GDM is increasing year by year. Currently, about 20% of pregnant women are diagnosed with GDM. GDM has great damage to both mother and fetus [Nat Rev Dis Primers.2019Jul 11;5(1):47.doi:10.1038 / s41572-019-0098-8.]. It is prone to infection, polyhydramnios, macrosomia, dystocia, birth canal injury, postpartum hemorrhage, increased cesarean section rate, fetal malformation, etc. Neonates are prone to neonatal respiratory distress syndrome, hypoglycemia, and hyperbilirubinemia. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K47/42A61K47/34A61K47/68A61K31/58A61K31/713A61K48/00A61K41/00A61P3/10A61P15/00A61P37/02
CPCA61K9/1271A61K9/1277A61K47/42A61K47/34A61K47/68A61K31/58A61K31/713A61K41/0052A61P3/10A61P15/00A61P37/02A61K2300/00
Inventor 郭宇
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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