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Composition for treating osteoarthritis or repairing cartilage injury and preparation method thereof

A technology for cartilage damage and osteoarthritis, applied in the biological field, can solve problems such as poor effect, and achieve the effect of shortening the treatment period, improving the effect and improving the treatment effect.

Pending Publication Date: 2022-03-25
武汉冉谷医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned technical deficiencies, provide a composition for treating osteoarthritis or repairing cartilage damage and its preparation method, and solve the poor effect of treating osteoarthritis or repairing cartilage damage in the prior art question

Method used

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  • Composition for treating osteoarthritis or repairing cartilage injury and preparation method thereof

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preparation example Construction

[0021] This specific embodiment also includes a kind of preparation method of above-mentioned composition, comprises the following steps:

[0022] Mix the second calcium chloride with platelet plasma at 15-30°C to obtain platelet-rich plasma; the platelet-rich plasma is obtained by the following steps: centrifuge the whole blood to obtain a serum layer, plasma layer and red blood cell layer, and take the plasma layer That is, the platelet plasma;

[0023] mixing the mesenchymal stem cells, the first calcium chloride and the thrombin solution to obtain a mixture;

[0024] The platelet-rich plasma is mixed with the mixture at 15-30° C. to obtain the mesenchymal stem cell preparation.

Embodiment 1

[0031] This example proposes a composition for treating osteoarthritis or repairing cartilage damage, which is obtained by mixing mesenchymal stem cells, platelet-rich plasma, the first calcium chloride and thrombin solution; the platelet-rich plasma includes the second Calcium chloride and platelet plasma; the concentration of the first calcium chloride in the mesenchymal stem cell preparation is 20 mg / mL, and the concentration of the second calcium chloride in the platelet-rich plasma is 70 mg / mL; The volume ratio of the mesenchymal stem cells to the platelet-rich plasma is 1:1; the volume ratio of the thrombin solution to the mesenchymal stem cells is 1:10.

[0032] The preparation method of the above-mentioned composition of the present embodiment, comprises the following steps:

[0033] Mix the second calcium chloride with platelet plasma at 15°C to obtain platelet-rich plasma; the platelet-rich plasma is obtained by the following steps: centrifuge the whole blood to obta...

Embodiment 2

[0037] This example proposes a composition for treating osteoarthritis or repairing cartilage damage, which is obtained by mixing mesenchymal stem cells, platelet-rich plasma, the first calcium chloride and thrombin solution; the platelet-rich plasma includes the second Calcium chloride and platelet plasma; the concentration of the first calcium chloride in the mesenchymal stem cell preparation is 30 mg / mL, and the concentration of the second calcium chloride in the platelet-rich plasma is 60 mg / mL; The volume ratio of the mesenchymal stem cells to the platelet-rich plasma is 2:1; the volume ratio of the thrombin solution to the mesenchymal stem cells is 1:15.

[0038] The preparation method of the above-mentioned composition of the present embodiment, comprises the following steps:

[0039] Mix the second calcium chloride with platelet plasma at 20°C to obtain platelet-rich plasma; the platelet-rich plasma is obtained by the following steps: centrifuge the whole blood to obta...

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Abstract

The invention discloses a composition for treating osteoarthritis or repairing cartilage injury and a preparation method thereof, and belongs to the technical field of biology. The composition for treating osteoarthritis or repairing cartilage injury is obtained by mixing mesenchymal stem cells, platelet-rich plasma, first calcium chloride and a thrombin solution, the platelet-rich plasma comprises second calcium chloride and platelet plasma; the concentration of the first calcium chloride in the mesenchymal stem cell preparation is 20-40 mg / mL, and the concentration of the second calcium chloride in the platelet-rich plasma is 60-70 mg / mL. The preparation method of the composition comprises the following steps: mixing second calcium chloride with platelet plasma at 15-30 DEG C to obtain platelet-rich plasma; mixing the mesenchymal stem cells, first calcium chloride and a thrombin solution to obtain a mixture; and mixing the platelet-rich plasma with the mixture to obtain the mesenchymal stem cell preparation. The composition improves the treatment effect and shortens the treatment cycle.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition for treating osteoarthritis or repairing cartilage damage and a preparation method thereof. Background technique [0002] Osteoarthritis is a degenerative disease, which is caused by aging, obesity, strain, trauma, congenital abnormalities of joints, joint deformities and many other factors. The disease is more common in middle-aged and elderly people, and it is more likely to occur in weight-bearing joints and joints with more activities (such as cervical spine, lumbar spine, knee joints, hip joints, etc.). Excessive weight bearing or overuse of these joints can promote degenerative changes. Clinical manifestations include slowly developing joint pain, tenderness, stiffness, joint swelling, limited mobility, and joint deformity. [0003] In addition, articular cartilage damage can also cause joint pain, swelling and dysfunction, seriously affecting the quality of lif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61P19/02A61P19/08A61P29/00A61K35/28A61K35/16A61K33/14A61K31/728
CPCA61K35/28A61K35/16A61K33/14A61K38/4833A61K31/728A61K9/0019A61P19/02A61P19/08A61P29/00C12Y304/21005A61K2300/00
Inventor 吴菲其他发明人请求不公开姓名
Owner 武汉冉谷医疗有限公司
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