PSMA-targeted macrophage membrane-coated bionic nano-drug carrier and application thereof

Abstract

The invention discloses a PSMA-targeted macrophage membrane-wrapped bionic nano-drug carrier and application, a nanogold carrier is wrapped by a macrophage membrane, and a PSMA-targeted antibody based on a sequence as shown in SEQ.ID.NO.1 is expressed on the macrophage membrane in a transmembrane manner; the antibody is a gy-1 single-chain antibody, and is expressed on a macrophage cell membrane in a transmembrane manner through fusion expression with a CD8a hinge region and a CD8a transmembrane region. The preparation method comprises the following steps: constructing a lentiviral vector for expressing a transmembrane single-chain antibody by utilizing a genetic engineering method, packaging into lentivirus, infecting macrophages to express the transmembrane single-chain antibody, and extracting a cell membrane to carry out bionic modification on a nano-vector; the prepared nano-carrier is expected to overcome the two problems of strong exogenous property and weak targeting property of the traditional nano-drug carrier, and can be used for preparing targeted diagnosis and treatment reagents and drugs for prostatic cancer.

Description

technical field [0001] The invention belongs to the technical field of biological targeting, relates to the specific targeting of prostate cancer, in particular to a macrophage membrane-wrapped biomimetic nano drug carrier targeting PSMA and its application. [0002] technical background [0003] Nanomedicine is considered to be one of the most promising anti-tumor strategies in the 21st century, and it has gradually become a hot spot and focus in the research and development of new tumor drugs. Among them, nano-drug carriers can not only increase the stability of drug delivery and improve the The water solubility of the drug; it is also expected that drugs with good anti-tumor activity but poor pharmacokinetics will be included in the candidate category, increasing the flexibility of clinical medication and providing new impetus for clinical diagnosis and treatment of tumors. However, traditional nano-drug delivery systems, such as FDA-approved liposomes, polymers, and album...

AI Technical Summary

Problems solved by technology

[0009] The present invention organically combines bionic nanotechnology, genetic engineering technology and antibody technology to construct a biomimetic nanomedicine carrier wrapped in macrophage cell membrane targeting PSMA, which overcomes the defects of strong exogenous and weak targeting of traditional nanomedicine carriers. Provide a new strategy for the targeted diagnosis and treatment of prostate cancer, and provide a new idea for the development of nano-drugs

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