Enterococcus faecium, application thereof, composition and fermentation culture method of enterococcus faecium

A technology of Enterococcus faecium and fermentation medium, applied in the field of microbial fermentation, can solve the problems of large differences in performance of Enterococcus faecium, not necessarily adapting to human or animal intestinal environment, and high cost

Pending Publication Date: 2022-04-12
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing Enterococcus faecium comes from commercially purchased industrial strains and standard strains, which are not necessarily suitable for the intestinal environment of humans or animals, and the performance of different strains of Enterococcus faecium varies greatly, so a strain with excellent comprehensive performance and Enterococcus faecium adapted to intestinal environment is of great importance
In the traditional fermentation production of Enterococcus faecium, MRS medium is mostly used for fermentation and cultivation, which has the disadvantage of high cost, and the production of Enterococcus faecium by traditional fermentation method has the problem of low viable count. Therefore, it is necessary to develop a method suitable for The fermentation culture method of Enterococcus faecium to reduce production costs and increase the yield of live bacteria is of great significance and value to promote the industrialization of Enterococcus faecium fermentation

Method used

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  • Enterococcus faecium, application thereof, composition and fermentation culture method of enterococcus faecium
  • Enterococcus faecium, application thereof, composition and fermentation culture method of enterococcus faecium
  • Enterococcus faecium, application thereof, composition and fermentation culture method of enterococcus faecium

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Embodiment 1

[0125] This embodiment provides a method for fermentation and cultivation of Enterococcus faecium HSC-18, wherein the fermentation scale is a 2-ton fermenter.

[0126] 1. Preparation of seed solution

[0127] (1) Seed activation

[0128] Take the Enterococcus faecium HSC-18 stored in the glycerol tube at -80°C. After the Enterococcus faecium HSC-18 glycerol tube is completely melted, draw 0.5mL of the bacterial liquid and inoculate it into 50mL of MRS liquid medium, and place it at 37°C under 160r Cultivate for 12 hours under the condition of 1 / min, then pick the bacterial solution and streak it on the MRS medium plate, place it at 37°C for 18 hours, and obtain a single colony of Enterococcus faecium HSC-18.

[0129] (2) Preparation of primary seed solution

[0130] A single colony of Enterococcus faecium HSC-18 was picked from the MRS medium plate, inoculated in 50 mL of MRS medium, and cultured in a shaker flask at 37°C and 160 r / min for 12 hours to obtain a first-grade se...

Embodiment 2

[0143] This embodiment provides a method for fermentation and cultivation of Enterococcus faecium HSC-18, wherein the fermentation scale is a 30L fermenter. The specific operation process is: according to the method in embodiment 1, prepare the primary seed liquid and the secondary seed liquid, and inoculate the secondary seed liquid into the fermenter of 30L according to the inoculum size of 1% by volume (the liquid capacity is 16L, the fermentation medium is the same as that in Example 1, and the actual elimination method of the fermenter is carried out with reference to Example 1), adjust the temperature of the tank to 37°C and stop stirring and aeration, and after keeping the pressure for 3 hours, Enterococcus faecium HSC-18 enters In the logarithmic growth phase, and the pH drops to 6.0; start stirring and control the rotating speed to 100r / min and micro-ventilation (see obvious bubbles), and add 40% (mass volume percentage, m / V) sodium hydroxide solution to control The p...

Embodiment 3

[0146] This embodiment provides a method for fermentation and cultivation of Enterococcus faecium HSC-18, wherein the fermentation scale is a 2-ton fermenter. Compared with the fermentation culture method of Example 1, the difference of the fermentation culture method of the present embodiment is: the fermentation medium is different, the fermentation medium of the present embodiment includes brown sugar of 10g / L, yeast extract of 12g / L , 10g / L fish peptone, 0.5g / L magnesium sulfate heptahydrate, 1g / L ammonium sulfate, 0.02g / L potassium dihydrogen phosphate, 0.1g / L sodium bicarbonate, 0.3g / L chlorine Potassium chloride and 0.5g / L light calcium carbonate.

[0147] In this example, the fermented liquid in the lower tank was diluted and coated on the MRS medium plate, and three parallel samples were set for each dilution, and counted after standing at 37° C. for 18 hours. Through counting, it can be known that The bacterial count was 8 billion CFU / mL.

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Abstract

The invention discloses enterococcus faecium, application and composition thereof and a fermentation culture method of the enterococcus faecium, the name of the enterococcus faecium is HSC-18, the classification name is Enterococcus faecium, the preservation number is CCTCC NO: M 20211446, the preservation date is November 18, 2021, the preservation unit is China Center for Type Culture Collection, the preservation number is CGMCC NO: M 20211446, and the preservation number is CGMCC NO: M 20211446. The enterococcus faecium can be used for preparing feed additives, animal feed, food additives, food or probiotics. The active component of the composition comprises enterococcus faecium HSC-18, and the composition is a feed additive, an animal feed, a food additive, food or a probiotic agent. The fermentation culture method of the enterococcus faecium comprises the following steps: inoculating the enterococcus faecium into an enterococcus faecium fermentation culture medium, and carrying out fermentation culture for 10-20 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 5.8-7.0.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to an Enterococcus faecium and its application, composition and a method for fermenting and cultivating the Enterococcus faecium. Background technique [0002] Enterococcus faecium (Enterococcus faecium) belongs to the Enterococcus genus. Enterococcus faecium is a facultative anaerobic Gram-positive bacterium, oval or club-shaped, without spores and without flagella. Because Enterococcus faecium can ferment and produce lactic acid, Enterococcus faecium belongs to lactic acid bacteria. Enterococcus faecium is a probiotic microorganism that exists in the intestinal tract of humans and animals. The lactic acid produced by the fermentation of Enterococcus faecium can effectively reduce the pH value of the intestinal environment and inhibit the growth of pathogenic bacteria; Bacterial substances, such as hydrogen peroxide, bacteriocin, etc., have a certain killing effect ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L29/00A23K10/18C12R1/01
Inventor 金梅林钟鸣杨欢欢缪鑫昕肖婉君张强
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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