Specific identification primer pair for turtle shells and decoction pieces, traditional Chinese medicine formula granules and other aqueous extract products thereof as well as application and identification method of specific identification primer pair

Abstract

The invention relates to a specific identification primer pair for turtle shells and decoction pieces, traditional Chinese medicine formula granules and other aqueous extract products thereof as well as application and an identification method of the specific identification primer pair. The specific identification primer pair comprises a forward primer with a base sequence as shown in SEQ ID NO: 1 and a reverse primer with a base sequence as shown in SEQ ID NO: 2. The specific identification primer pair is adopted to amplify the DNA of the turtle shell water extract product, and the obtained amplification product not only can be used for identifying the turtle shell and the decoction pieces thereof, but also can be used for identifying the traditional Chinese medicine standard decoction of the turtle shell and the decoction pieces thereof and other water extract products, so that the turtle shell and the decoction pieces thereof are distinguished from counterfeit water extract products; the problem that the water extract product is difficult to identify after losing the form is solved. When the specific identification primer pair is used for identification, the specificity is good, the result accuracy is high, and a guarantee is provided for the medication safety and the clinical curative effect of traditional Chinese medicine standard decoction of the turtle shell and decoction pieces thereof and other water extract products.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a pair of specific identification primers for turtle shells and their decoction pieces, traditional Chinese medicine formula granules and other water extract products, and their application and identification method. Background technique [0002] According to the 2020 edition of the Chinese Pharmacopoeia, the turtle shell is derived from the carapace of the soft-shelled turtle (Trionyx sinensis Wiegmann). Turtle shell, first recorded in the "Shen Nong's Materia Medica" in the Han Dynasty, is listed as a middle grade. It has been recorded in successive dynasties of herbal medicines. It has the effects of nourishing yin and subsidizing yang, reducing fever and steaming, softening and resolving hard masses, and is often used for fever due to yin deficiency and steamy bones. Heat, deficiency of yin and hyperactivity of yang, dizziness, inter...

AI Technical Summary

Problems solved by technology

[0003] Turtle shell is a commonly used traditional Chinese medicine. It is more common to use the same genus and its counterfeit products as turtle shell in the market, such as Chinemys reevesii (Gray), Apalone spinifera (Apalone spinifera), Florida soft-shelled turtle (Apaloneferox), Trionyx steindachneri, Lissemys punctata, Trionyx hurum, Flat soft-shelled turtle (Dogania subplana), Pelochelys bibroni, Trionyx gangeticus) A, African soft-shelled turtle (Trionyx triunguis) A, etc., they are close in character and shape, relying on the traditional identification method is difficult and the identification accuracy is low, especially after the vinegar is made into the processed product, and the water extraction is made into the standard of traditional Chinese medicine. After the decoction is further processed into traditional Chinese medicine formula granules, it is more difficult to carry out effective identification due to the loss of medicinal properties

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