Compound lactic acid bacteria, fermented milk and application of fermented milk in relieving constipation

A technology of complex lactic acid bacteria and fermented milk, applied in the direction of application, lactobacillus, bacteria, etc., can solve problems such as excessive flatulence

Pending Publication Date: 2022-04-19
HEILONGJIANG FEIHE DAIRY +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term ingestion of laxatives can cause some adverse effects such as abdominal cramps, skin rashes, excessive gas and dizziness

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound lactic acid bacteria, fermented milk and application of fermented milk in relieving constipation
  • Compound lactic acid bacteria, fermented milk and application of fermented milk in relieving constipation
  • Compound lactic acid bacteria, fermented milk and application of fermented milk in relieving constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation of the strain of Example 1

[0046] Lactobacillus paracasei (Lactobacillus paracasei) DPUL-40, Lactobacillus paracasei (Lactobacillus paracasei) DPUL-44 and Lactobacillus Germani Bulgarian subspecies (Lactobacillusdelbruedkii subsp. Bulgaricus) DPUL-36 is isolated from traditional fermented milk in Xinjiang. Lactobacillus paracasei DPUL-40's 16SrDNA as shown in SEQ ID NO.2, Lactobacillus paracasei's 16SrDNA from DPUL-44 as shown in SEQ ID NO.3, Lactobacillus delbruedkii subsp. Bulgaricus) DPUL-36's 16SrDNA is shown in SEQ ID NO.1.

 [0047] The strains are preserved in the China Typical Culture Preservation Center (CCTCC, address: Luojia Mountain, Wuchang, Wuhan, China Typical Culture Preservation Center, Zip Code 430072). Lactobacillus paracasei DPUL-40 has a culture reserve number of CCTCC NO:M2021836, the preservation date is July 8, 2021, the classification is named Lactobacillus paracasei, and the strain name is DPUL-40. Lactobacillusparacasei DPUL-44 has a st...

Embodiment 2

[0048] Example 2 safety determination of strains

[0049] The safety determination of the three strains of the present invention is as follows:

[0050] (1) Hemolytic

[0051] Activated Lactobacillus paracasei DPUL-40, Lactobacillus paracasei DPUL-44 and Lactobacillus Germani Bulgarian subspecies DPUL-36 scribing were inoculated in Solid Medium of Columbia for 48 h at 37 °C. If there is a transparent circle around the colony, it has β-hemolysis, and if there is no transparent ring, it has γ-hemolyticity, where the strain with β-hemolytic is usually pathogenic. Listeria monocytogenes was used as a positive control. See Attached for details Figure 4 。 It can be seen that there is a clear circle around the colony of Listeria monocytogenes, while there is no transparent circle around the three strains of lactic acid bacteria, which proves that the three strains are not hemolytic and are a relatively safe strain.

[0052] (2) Antibiotic sensitivity

[0053] According to the standard me...

Embodiment 3

[0071] Example 3 of the present invention compound lactic acid bacteria fermentation milk storage stability

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses compound lactic acid bacteria, fermented milk and application in relieving constipation, and belongs to the technical field of microorganism application. The compound lactic acid bacteria comprise lactobacillus paracasei DPUL-40, lactobacillus paracasei DPUL-44 and lactobacillus delbrueckii subsp. Bulgaricus DPUL-36, and the strain preservation number of the lactobacillus paracasei DPUL-40 is CCTCC (China Center for Type Culture Collection) NO: M2021836; the strain preservation number of the lactobacillus paracasei DPUL-44 is CCTCC (China Center For Type Culture Collection) NO: M2021837; the strain preservation number of the lactobacillus delbrueckii subsp. Bulgaricus DPUL-36 is CCTCC (China Center For Type Culture Collection) NO: M2021835. The compound lactic acid bacteria or the prepared compound lactic acid bacteria fermented milk can be used for relieving constipation caused by loperamide, increasing the number of excrement particles and the water content of excrement, shortening the first-particle black excrement discharging time, improving the gastrointestinal transmission rate and improving the gastrointestinal motility function.

Description

Technical field [0001] The present invention belongs to the field of microbial application technology, specifically relates to a compound lactic acid bacteria, fermented milk and applications in the mitigation of constipation. Background [0002] Fermented milk is a dairy product made by fermenting lactic acid bacteria with fresh cow's milk, goat's milk or reconstituted milk as raw materials. A large number of research results show that fermented milk has important physiological functions such as adjusting intestinal flora, lowering cholesterol, enhancing the body's immunity, and promoting gastrointestinal absorption. Traditional natural fermented dairy products contain rich lactic acid bacteria, some scholars have counted researchers in recent years from traditional fermentation products isolated as many as 3388 species of lactic acid bacteria, Maino. Mutula studied the "Sailimum" yogurt produced in Baicheng, Xinjiang, isolated two strains of extracellular polysaccharide-produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20A23C9/123A23L33/135C12R1/225
CPCC12N1/20A23C9/1238A23C9/1234A23L33/135A23V2002/00A23V2400/165A23V2400/123A23V2200/3204
Inventor高鹏蒋士龙郭华妥彦峰牟光庆
OwnerHEILONGJIANG FEIHE DAIRY