Bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and application of bifidobacterium breve

A technology of bifidobacteria breve and microbial inoculum, applied in the field of microorganisms, can solve the problems of single function of bifidobacteria and some inconspicuous functions, and achieve the effect of improving the water content of feces and maintaining good vitality

Active Publication Date: 2022-07-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on bifidobacteria for relieving constipation will have a huge impact on food science, microbiology, and preventive medicine. Due to the single function of bifidobacteria that has the effect of relieving constipation in the prior art and some functions are relatively small Not outstanding, therefore, research on the effect of bifidobacteria on constipation relief is needed

Method used

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  • Bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and application of bifidobacterium breve
  • Bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and application of bifidobacterium breve
  • Bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and application of bifidobacterium breve

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Acquisition of Bifidobacterium breve (B. breve) CCFM1260

[0066] Specific steps are as follows:

[0067] 1. Isolation and screening of Bifidobacterium strains:

[0068] (1) The feces of 9-month-old males in Beijing were collected using a disposable sterile urinal, and the fecal samples were placed in a liquid medium containing MRS+mass percent (0.05%-0.1%) cysteine ​​containing fructooligosaccharides, and the Anaerobic incubator (N 2 :CO 2 :H 2 =80:10:10) for 12h enrichment;

[0069] (2) The fecal sample was diluted with sterile physiological saline and then applied to MRS+ mass percentage (0.05%-0.1%) L- On the solid plate of cysteine ​​hydrochloride, cultivate for 24-48h;

[0070] (3) choose the single colony that meets the basic form of bifidobacteria to carry out streak purification on the plate, and screen and isolate the selected strain of bifidobacteria;

[0071] (4) The above single colony was cultured in liquid MRS+mass percentage (0.05%-0.1%...

Embodiment 2

[0090] Example 2: Bifidobacterium breve (B. breve) CCFM1260 relieves constipation-related symptoms induced by loperamide

[0091] Specific steps are as follows:

[0092] (1) Preparation of Bifidobacterium breve CCFM1260

[0093] Bifidobacterium breve CCFM1260 strains were taken out from the -80°C refrigerator, streaked in MRS solid medium, cultured anaerobic at 37°C for 48h, and a single colony was picked in MRS liquid medium, anaerobic culture at 37°C for 24h, prepared seed solution;

[0094] The prepared seed liquid was inoculated into a new MRS liquid medium with an inoculum of 2% (v / v), cultured anaerobic at 37°C for 24h, and cultured for another generation in the same way to prepare Bifidobacterium breve CCFM1260 fermentation broth;

[0095] Then the prepared Bifidobacterium breve CCFM1260 fermentation broth was centrifuged at 6000 r / min and 4°C for 5 min, and resuspended with 10% skim milk to obtain a bacterial suspension, which could be used in animal experiments.

...

Embodiment 3

[0107] Example 3: Bifidobacterium breve (B. breve) CCFM1260 down-regulates the content of interleukin 17 (IL-17) in colon tissue of constipated mice

[0108] (1) The methods of grouping, modeling and treatment of BABL / c mice were the same as those in Example 2.

[0109] (2) After the mice were sacrificed on the 36th day, the collected mouse colon tissue was crushed and ground to make a homogenate, centrifuged at 12,000 g for 15 min to take the supernatant, and the mouse interleukin 17 detection kit was used, and the procedure was carried out according to the instructions. In the experiment, the concentration of interleukin 17 in colon tissue was calculated from the standard curve, and the total protein content of colon tissue was measured by BCA total protein kit, and the final result was the ratio of interleukin 17 to total protein concentration.

[0110] The experimental results are as figure 2 shown, by figure 2 It can be seen that after loperamide was used for modeling...

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Abstract

The invention discloses bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and application of the bifidobacterium breve, and belongs to the field of microorganisms. The Bifidobacterium breve (B.breve) CCFM1260 provided by the invention can effectively relieve the first black stool time, the intestinal tract propulsion rate and the water content of stool of a constipation mouse, and has the effect of obviously improving the apparent pathological indexes of constipation; excellent benzopyrene adsorption capability is realized; in the detection of inflammation indexes, the bifidobacterium breve has a remarkable down-regulation effect on proinflammatory factors IL-17 and IL-1beta, and is more suitable for constipation patients accompanied by intestinal inflammation.

Description

technical field [0001] The invention relates to a Bifidobacterium breve capable of down-regulating IL-17 and relieving constipation and its application, belonging to the field of microorganisms. Background technique [0002] With the change of diet structure and the change of life rhythm, the number of patients with constipation is increasing. Although constipation does not directly threaten people's life safety, the harm caused by this disease cannot be ignored. Specifically: constipation can aggravate the original digestive tract symptoms, such as further disturbance of digestive function, leading to and aggravating anorectal diseases, and long-term constipation may lead to colon cancer; constipation patients can induce cardiovascular and cerebrovascular events through forced defecation; some intestinal metabolism Products can affect brain function; patients with constipation have significantly reduced quality of life, and patients may experience mood disturbances. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A61P1/00A61P29/00A23L33/135C12R1/01
CPCC12N1/20A61K35/745A61P1/10A61P1/00A61P29/00A23L33/135Y02A50/30
Inventor 王琳琳李亦汉王刚赵建新张灏陈卫
Owner JIANGNAN UNIV
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