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A Bifidobacterium bifidum that relieves constipation and regulates intestinal flora disturbance and its application

A technology for bifidobacteria bifidum and constipation, applied in the field of microorganisms, can solve problems such as insignificant curative effect, and achieve the effects of improving diversity, restoring health, and improving the advancement rate of the small intestine.

Active Publication Date: 2022-07-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, Wang Junwen et al. pointed out in "Research Progress in the Treatment of Functional Constipation and Constipation-Predominant Irritable Bowel Syndrome" that bifidobacteria have no obvious curative effect on chronic constipation.

Method used

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  • A Bifidobacterium bifidum that relieves constipation and regulates intestinal flora disturbance and its application
  • A Bifidobacterium bifidum that relieves constipation and regulates intestinal flora disturbance and its application
  • A Bifidobacterium bifidum that relieves constipation and regulates intestinal flora disturbance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1: Acquisition of Bifidobacterium bifidum CCFM1166

[0114] Specific steps are as follows:

[0115] 1. Isolation and screening of Bifidobacterium strains:

[0116] (1) Use a disposable sterile urinal to collect fecal samples of male infants who were breastfed by natural delivery in Suzhou City, Jiangsu Province, and put the fecal samples in a liquid containing MRS + mass percentage (0.05%-0.1%) cysteine In the medium, enriched in an anaerobic incubator (N2:CO2:H2=80:10:10) for 12h;

[0117] (2) The fecal sample was diluted with sterile physiological saline and then applied to MRS+ mass percentage (0.05%-0.1%) L- On the solid plate of cysteine ​​hydrochloride, cultivate for 24-48h;

[0118] (3) Select a single colony that conforms to the basic form of bifidobacteria to carry out streak purification on a plate, and screen and isolate the selected strain of bifidobacteria;

[0119] (4) The above single colony was cultured in liquid MRS+mass percentage (0.05%-0....

Embodiment 2

[0138] Example 2: Relief of loperamide-induced constipation-related symptoms by Bifidobacterium bifidum CCFM1166

[0139] Specific steps are as follows:

[0140] (1) Preparation of Bifidobacterium bifidum CCFM1166 bacterial suspension

[0141] Bifidobacterium bifidum CCFM1166 strain was taken out from the -80°C refrigerator, streaked in MRS solid medium, cultivated at 37°C for 48h, picked a single colony in MRS liquid medium, and cultivated at 37°C for 24h to prepare seeds liquid;

[0142] The prepared seed liquid was inoculated into a new MRS liquid medium with an inoculum of 2% (v / v), cultivated at 37 ° C for 24 hours, and cultured for another generation in the same way to prepare Bifidobacterium bifidum CCFM1166 fermentation liquid;

[0143] Then the prepared Bifidobacterium bifidum CCFM1166 fermentation broth was centrifuged at 6000 r / min and 4°C for 5 min, and then resuspended with 10% (w / v) skim milk to obtain a bacterial suspension, for animal experiments.

[0144]...

Embodiment 3

[0156] Example 3: Bifidobacterium bifidum CCFM1166 can significantly reduce the content of somatostatin (SS) in the serum of constipated mice

[0157] Specific steps are as follows:

[0158] (1) The grouping, modeling and treatment methods of C57BL / 6J mice were the same as those in Example 2.

[0159] (2) After the mice were sacrificed on the 40th day, the collected blood of the mice was allowed to stand for 2 hours, and the serum was obtained after centrifugation at 3000 × g for 15 minutes. The somatostatin (SS) detection kit was used. The concentration of SS in serum was calculated.

[0160] Studies have shown that somatostatin, as a neurohormone with a wide range of effects, can inhibit the release of various gastrointestinal hormones, such as gastrin, motilin, glucagon, and calcitonin. Therefore, it has a certain inhibitory effect on gastrointestinal motility.

[0161] The experimental results are as figure 2 shown, by figure 2 It can be seen that compared with the ...

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Abstract

The invention discloses a bifidobacteria bifidum for relieving constipation and regulating intestinal flora disorder and its application, belonging to the field of microorganisms. The bifidobacteria bifidum provided by the invention can significantly improve the water content of the feces and the propulsion rate of the small intestine, reduce the time of the first black stool in the constipated mice, and can obviously improve the apparent pathological indicators of constipation; in the detection of physiological indicators, the two Bifidobacterium can also increase the content of motilin, reduce the content of somatostatin, and fundamentally affect intestinal peristalsis; Bifidobacterium can regulate the abundance of important microorganisms in the intestine and maintain intestinal homeostasis; two Bifidobacterium CCFM1166 is better than GDMCC NO: 60940 to increase the content of MTL in serum, reduce the content of SS in serum, and has a more significant effect on promoting small intestinal peristalsis. Therefore, in the future, more personalized medicine can be used for patients with constipation. The treatment plan has very considerable application prospects.

Description

technical field [0001] The invention relates to a bifidobacteria bifidum for relieving constipation and regulating intestinal flora disorder and its application, belonging to the field of microorganisms. Background technique [0002] As we all know, constipation is a kind of global disease with a very high prevalence and a wide range. At present, constipation has appeared in all age groups around the world, which has troubled many people. Therefore, for The treatment methods related to constipation need more attention and attention. [0003] There are many reasons for constipation, such as disturbance of daily routine, unbalanced diet, side effects caused by some drugs, and even complications caused by some diseases. These different causes may also bring different symptoms of constipation. Therefore, constipation should be distinguished according to different classification methods. According to the onset mode, it can be divided into acute constipation and chronic constipa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123A23C13/16A23C19/032A23C19/06A23L11/50A23L11/65A23L33/135A61K35/741A61P1/10A61P1/00C12R1/01
CPCA23C9/1234A23C13/16A23C19/0323A23C19/062A23C11/106A23L33/135A61K35/741A61P1/10A61P1/00A23V2002/00A23V2400/517A23V2200/3204
Inventor 王琳琳王刚柴茂赵建新陈卫张灏
Owner JIANGNAN UNIV
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