Bifidobacterium longum subsp. longum for relieving constipation and increasing total bile acid content of excrement and application of Bifidobacterium longum subsp. longum

A technology of Bifidobacterium longum and subspecies, applied in the field of microorganisms, can solve problems such as single function, difficult activation, and slow growth, and achieve good growth characteristics, avoid side effects, and obvious effects

Active Publication Date: 2022-01-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are certain limitations (such as slow growth, difficulty in activation, single function, etc.) in the bifidobacteria with the effect of relieving constipation faced by the prior art. The purpose of the present invention is to provide a strain that is easy to activate and has a short growth cycle. Bifidobacterium longum that increases the total bile acid content in feces and effectively relieves constipation, and has a certain effect on improving the bioavailability of curcumin in the body, can overcome the deficiencies in the prior art, and provide corresponding probiotic preparations, functional food, thereby preventing constipation, or enabling constipation patients to gradually get rid of the side effects and limitations of drug treatment

Method used

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  • Bifidobacterium longum subsp. longum for relieving constipation and increasing total bile acid content of excrement and application of Bifidobacterium longum subsp. longum
  • Bifidobacterium longum subsp. longum for relieving constipation and increasing total bile acid content of excrement and application of Bifidobacterium longum subsp. longum
  • Bifidobacterium longum subsp. longum for relieving constipation and increasing total bile acid content of excrement and application of Bifidobacterium longum subsp. longum

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0073] (4) Preparation of Bifidobacterium longum suspension

[0074] Inoculate the 3-generation activated bacterial solution into 1L of liquid MRS medium at an inoculum size of 2%, shake and mix well, and incubate in an anaerobic incubator at 37°C for 24h. Centrifuge at 8000g / min for 15min at 4°C, remove the supernatant, wash twice with sterile saline containing 0.05%-0.1% L-cysteine ​​hydrochloride, and centrifuge under the same conditions. After the supernatant was removed, it was stored in 30% glycerol and stored in a -80°C refrigerator for one week. Before the animal experiment, centrifuge the bacterial solution at 6000r / min for 5min, wash it twice with sterile saline, resuspend the bacterial solution with 10% skimmed milk, shake it evenly, and use the plate pouring method to measure the initial and one week after freezing the number of live bacteria. The formula of MRS medium is: beef extract 10g; tryptone 10g; yeast powder 5g; glucose 20g; anhydrous sodium acetate 5g; ...

Embodiment 1

[0076] Example 1: Bifidobacterium longum CCFM1113 relieves constipation-related symptoms induced by loperamide

[0077] Bifidobacterium longum CCFM1113, Bifidobacterium bifidum CGMCC NO.13632 (the strain is disclosed in the patent document CN106834187B) and Bifidobacterium longum CCFM642 (the strain is disclosed in the paper DOI: 10.3389 / fmicb.2019.01721 Middle) strains were taken out from the -80°C refrigerator, streaked on the MRS plate, cultured at 37°C for 48 hours, picked a single colony in the MRS liquid tube, cultured at 37°C for 24 hours, and inoculated on a new MRS at a volume of 2%. In liquid culture medium, cultivate at 37°C for 24 hours, culture for another generation in the same way, then centrifuge the bifidobacterium suspension at 6000r / min, 4°C for 5min, and then resuspend with 10% skimmed milk, Bacterial suspensions were obtained for animal experiments.

[0078] Thirty 6-week-old healthy male Balb / c mice were taken, adapted to the environment for 1 week, and ...

Embodiment 2

[0087] Embodiment 2: Bifidobacterium longum CCFM1113 can significantly improve the content of total bile acid (TBA) in feces of mice with constipation

[0088] Balb / c mice grouping, modeling and treatment methods were the same as in Example 1. The feces collected and freeze-dried on the 37th day were pre-treated: take 20 mg of dried mouse feces, add 1 mL of tert-butanol solution (volume ratio 1:100), vortex and mix, and then vortex after 15 minutes in a 37°C water bath Mix well for 10 seconds, and finally centrifuge at 4°C, 10,000×g for 2 minutes to take the supernatant. The total TBA content in the supernatant was determined by using the total TBA kit according to the relevant content of the instructions.

[0089] Total bile acids (TBA) are a group of metabolites of cholesterol in the liver and entero-hepatic circulation, and are mainly divided into two categories: primary bile acids and secondary bile acids. Changes in total bile acid content indicate to a certain extent t...

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Abstract

The invention discloses Bifidobacterium longum subsp. longum for relieving constipation and increasing the total bile acid content of excrement and application of the bifidobacterium longum subsp. Longum, and belongs to the field of microorganisms. The Bifidobacterium longum provided by the invention can be used for remarkably increasing the content of the total bile acid in excrement, effectively relieving the first black excrement time, the intestinal tract propulsion rate and the water content of excrement of a mouse with constipation, and has the effect of remarkably improving the apparent pathological indexes of constipation; in the detection of physiological indexes, the Bifidobacterium longum can also influence the content of some neuropeptides, so that the symptoms of constipation are fundamentally influenced; and the Bifidobacterium longum not only has a certain considerable effect in physiological and pathological aspects and has no side effects compared with laxative, but also can improve the bioavailability of curcumin in vivo, so that the curcumin can better exert the anti-inflammatory and anti-oxidation effects, and therefore, the Bifidobacterium longum subsp. longum has a very considerable application prospect in the aspect of preparing functional foods or medicines.

Description

technical field [0001] The invention relates to a bifidobacterium longum subsp. longum which relieves constipation and increases the total bile acid content of feces and its application, belonging to the field of microorganisms. Background technique [0002] At present, constipation is a disease that has appeared in all age groups around the world. It is a global disease with a high prevalence and a wide range. , Therefore, whether it is for different countries or different ethnic groups, this is an important issue that needs to be resolved urgently. [0003] Constipation is mainly manifested as low defecation frequency, less than 3 times a week, dry stool, feeling of endless defecation, and even needs fingers to assist defecation. The high prevalence of constipation is often inseparable from the causes of constipation, such as work and rest disorders, unbalanced diet, microbial disorders, side effects of drugs, complications caused by metabolic diseases such as diabetes, g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12A23C13/16A23C19/06A23L11/50A23L11/65A23L2/38A23L2/84A23L29/00A23L33/135A23L19/00A61K35/745A61P1/10C12R1/01A61K31/352
CPCA23C9/1234A23C13/16A23C19/062A23C9/16A23C11/106A23L2/382A23L2/84A23L29/065A23L33/135A23L19/00A61K35/745A61K31/352A61P1/10A23V2002/00A23V2400/533A23V2200/30A23V2200/3204A61K2300/00Y02A50/30
Inventor 赵建新王刚柴茂陈卫张灏
Owner JIANGNAN UNIV
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