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Application of long non-coding RNA in diagnosis and treatment of tongue squamous carcinoma

A technology of tongue squamous cell carcinoma and RNA interference, which is applied in the field of herbal medicine and molecular biology, and can solve the problem of less LncRNA

Pending Publication Date: 2022-04-19
山东杰凯生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few relevant studies on the role of lncRNA in tongue squamous cell carcinoma, so it is necessary to explore more effective lncRNA as markers and targets for clinical diagnosis and treatment detection of tongue squamous cell carcinoma

Method used

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  • Application of long non-coding RNA in diagnosis and treatment of tongue squamous carcinoma
  • Application of long non-coding RNA in diagnosis and treatment of tongue squamous carcinoma
  • Application of long non-coding RNA in diagnosis and treatment of tongue squamous carcinoma

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Experimental program
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Effect test

Embodiment

[0114] 1. Materials and methods

[0115] 1. Experimental materials

[0116] 1.1 Gene information

[0117] species gene name Gene ID Gene full name human LINC01356 100996702 long intergenic non-protein coding RNA 1356

[0118] 1.2 Cell information: Human tongue squamous cell carcinoma cells CAL-27 and SCC9 were obtained from the Cell Bank of Shanghai Chinese Academy of Sciences.

[0119] cell name Cell Chinese name source species CAL-27 tongue squamous cell carcinoma people SCC9 tongue squamous cell carcinoma people

[0120] 2. Main reagents

[0121] 2.1 Purchasing reagents

[0122] 1) DMEM culture medium was purchased from HYCLONE Company of the United States.

[0123] 2) Serum was purchased from Gibco.

[0124] 3) Double antibodies, 0.25% trypsin digestion solution, and CCK-8 reagent were purchased from Beijing Suo Laibao Company.

[0125] 4) Lipofectamine2000 liposomes were purchased from Invitrogen, US...

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Abstract

The invention provides application of long non-coding RNA (Ribonucleic Acid) in diagnosis and treatment of tongue squamous cell carcinoma, and belongs to the technical field of crude drugs and molecular biology. It is proved for the first time that LINC01356 expression is increased along with increase of tongue squamous cell carcinoma malignancy degree and is in negative correlation with the survival rate. After the expression of the LINC01356 is interfered, the proliferation of tongue squamous carcinoma cells is inhibited, the apoptosis of the tongue squamous carcinoma cells is promoted, and the LINC01356 can be used as an effective medicine for preventing and / or treating the tongue squamous carcinoma. The invention provides a more favorable means for diagnosis and prognosis evaluation analysis of tongue squamous cell carcinoma, which is of great significance for research and treatment of tongue squamous cell carcinoma. Meanwhile, an experimental foundation is laid for developing high-efficiency related medicines for treating tongue squamous cell carcinoma, and a new view field is provided, so that the application has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of crude drug medicine and molecular biology, and specifically relates to the application of long non-coding RNA in the diagnosis and treatment of squamous cell carcinoma of the tongue. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tongue squamous cell carcinoma (TSCC), referred to as tongue squamous cell carcinoma, is one of the most common malignant tumors in the oral and maxillofacial region. It is highly malignant, highly invasive, and prone to cervical lymph node metastasis. Despite significant advances in prevention and treatment, the survival rate of TSCC patients remains poor. The results showe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K45/00A61P35/00A61P35/04
CPCC12Q1/6886A61K45/00A61P35/00A61P35/04C12Q2600/118C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 赵凯孙美玉李森朋刘爽田晓婷李倩
Owner 山东杰凯生物科技有限公司
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