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Isolation of chromosome carotenoids from fruits

A technology of carotene and chromoplasts, applied in the field of carotenoid production

Active Publication Date: 2022-04-26
UNIV DEGLI STUDI DI CAGLIARI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current methods related to capsicum carotenoids only target the carotenoid capsanthin with yields between 15% and 50% (CN1740236A)

Method used

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  • Isolation of chromosome carotenoids from fruits
  • Isolation of chromosome carotenoids from fruits

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Embodiment Construction

[0018] The present invention relates to a method for obtaining chromoplast carotenoids which can be isolated from plants, in particular the fruits of plants. By chromoplast carotenoids we mean all carotenoids that accumulate in different forms (free, bound to sugars or bound to proteins) in fruit-specific plastid organelles called chromoplasts. Among the long list of carotenoids found in chromoplasts, depending on the type of fruit, one can find: capsanthin, lycopene, carotenoids (-alpha, -beta, -gamma) and lutein, e.g. maize flavin, violaxanthin and lutein.

[0019] In more detail, the method according to the invention allows the selective separation of carotenoids such as capsanthin in the form of aggregates whose shape and size are regular based on size (size exclusion chromatography) and in pure form. The method includes the following steps:

[0020] i) Suspension production of uniform colored film fragments from fruit such as that of sweet pepper (common red pepper) at ...

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Abstract

The present invention relates to a method for producing carotenoids that can be isolated from fruits. The process of the present invention allows the selective separation of carotenoids in aggregate form of regular shapes and sizes based on size (size exclusion chromatography) and pure form. The method comprises the following steps: i) producing homogeneous plastid pigment film fragments from the fruit in a suspension; ii) solution separation of the membrane components by dissolution with a detergent; iii) the carotenoids in the form of regular sub-crystalline aggregates are selectively and highly pure separated by a screening technique, such as size exclusion chromatography (SEC-size exclusion chromatography).

Description

technical field [0001] The present invention relates to a process for the production of carotenoids, in particular to the industrial isolation of chromoplastid carotenoids which can be isolated from fruits containing chromoplastid carotenoids. For example, we can specifically mention the carotenoid capsanthin present in the red (red pepper) fruit of the sweet pepper (Capsicum annuum), the carotenoid leaf flavin, and the carotenoid lycopene present in the tomato (Solanum lycopersicum) (tomato). Equivalent procedures can be used to isolate other carotenoids present in the chromoplasts of other varieties of these fruits (e.g. capsicum and yellow tomato) and can in any case be extended to produce carotenoid accumulation at the chromoplast level as Any plant species characterized by fruit. [0002] The process allows you to: [0003] - Isolation of specific carotenoids from natural sources (not by synthetic methods); [0004] - Substantial increase in production supply (limitin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/34A23L5/44A23L33/105C12P23/00C07C403/24B01D11/02C12M1/26B01D61/14
CPCB01D15/34A23L33/105C07C403/24A23V2250/211A23V2002/00A23L5/44C07C2601/14C07C2601/16C07C45/78C07C2601/08C07C49/21C07C7/005C07C7/10C07C7/12C07C45/79
Inventor D·皮亚诺D·法尔奇
Owner UNIV DEGLI STUDI DI CAGLIARI