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Application of temperature-sensitive hydrogel packaging mitochondria

A temperature-sensitive hydrogel and mitochondrial technology, applied in the field of biomedicine, to achieve the effect of improving energy metabolism and mechanical contraction and relaxation functions, delaying heart failure, important clinical promotion and application value

Pending Publication Date: 2022-05-13
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problem of how to transplant mitochondria to treat myocardial ischemia-reperfusion injury

Method used

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  • Application of temperature-sensitive hydrogel packaging mitochondria
  • Application of temperature-sensitive hydrogel packaging mitochondria
  • Application of temperature-sensitive hydrogel packaging mitochondria

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Experimental program
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Effect test

Embodiment

[0022] 1. The Pluronic F127 powder (Sigma Company,) was formulated into a 15% (w / w%) hydrogel with physiological saline, and stored at low temperature.

[0023] 2. Obtain the myocardium of C57BL / 6 mice, digest the myocardium with enzymes and resuspend the mitochondrial isolation reagent using the mitochondrial isolation kit (Beyond Biotechnology Co., Ltd., C3606), grind it appropriately, centrifuge at 1000g, 4°C for 5 minutes, and take out Clear, transfer to a new EP tube, continue to centrifuge (3500g, 4°C, 10min) to obtain purified mitochondria, use 15% w / w Pluronic F127 thermosensitive hydrogel to resuspend in a low temperature environment of 0-4°C, shake slowly Mix well for 5-10min and set aside. All of the above are performed aseptically.

[0024] 3. Prepare the myocardial ischemia-reperfusion injury model of mice (ischemia for 45min, reperfusion for 24 hours), and inject 25ul microliters of hydrogel-encapsulated mitochondria (5 -10×10 4 sham operation in the control g...

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Abstract

The invention relates to application of temperature-sensitive hydrogel in packaging mitochondria, and belongs to the technical field of biological medicine. The invention provides application of temperature-sensitive hydrogel encapsulated mitochondria in preparation of a medicine for preventing or treating myocardial injury and application of temperature-sensitive hydrogel encapsulated mitochondria in preparation of a medicine for preventing or treating myocardial ischemia-reperfusion injury. The invention discloses application of a temperature-sensitive hydrogel encapsulated mitochondria as an in-vivo graft. The temperature-sensitive hydrogel is conveyed to a target area by packaging mitochondria and is applied to preparation of preventive or therapeutic drugs. According to the invention, the hydrogel with excellent hydrophilic performance and biocompatibility is used as a carrier for mitochondrial transplantation, so that the mitochondrial uptake rate of myocardial cells is increased, the energy metabolism and mechanical contraction and relaxation functions of ischemic myocardial cells are improved, and the occurrence of heart failure is delayed. The hydrogel can be overlaid to improve the damaged myocardial mechanical microenvironment and the mitochondrial transplantation effect, and the hydrogel has important clinical popularization and application value.

Description

technical field [0001] The invention relates to the application of temperature-sensitive hydrogel to encapsulate mitochondria, and belongs to the technical field of biomedicine. Background technique [0002] Mitochondria are the main power plant for eukaryotic cells to obtain energy support. As a high-energy-consuming organ, the heart is particularly dependent on mitochondrial metabolism. Myocardial ischemia and reperfusion injury are the key clinical problems to be solved urgently. The survival rate of myocardial cells directly determines the size of myocardial infarction and the function and degree of deterioration of the heart after repair. Many current studies have confirmed that mitochondria with complete structure and activity Transplantation can inhibit myocardial damage and improve cardiac function. 80% of exogenous mitochondria will be quickly absorbed and internalized by cardiomyocytes in a short period of time, improving the respiratory function of cells and incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K35/34A61P9/00A61P9/10
CPCA61K9/06A61K35/34A61P9/00A61P9/10
Inventor 孙爱军葛均波黄涯孙晓垒高日峰陈航
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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