Synthetic method of LDH@SiO2 shell-nuclear nano composite material

A technology of nanocomposite materials and synthesis methods, which is applied in the field of synthesis of LDHSiO2 shell-core nanocomposite materials, can solve the problems of difficult insertion of plasmid DNA, limited DNA carrying capacity, and weakened plasmid protection effect, achieving huge application potential and benefiting The effect of DC recognition and prolongation of antigen retention time

Active Publication Date: 2015-01-28
HEILONGJIANG UNIV
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Problems solved by technology

[0004] However, LDH also has some shortcomings as a gene delivery carrier, such as the small distance between layers, and it is difficult to insert macromolecular plasmid DNA into it; if the plasmid DNA is adsorbed on the surface of LDH, the protective effect on the plasmid will be weakened, and the DNA carrying capacity It is also very limited, which makes LDH as a gene delivery vehicle have a good protective effect in vivo, but the therapeutic effect is not ideal

Method used

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  • Synthetic method of LDH@SiO2 shell-nuclear nano composite material
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  • Synthetic method of LDH@SiO2 shell-nuclear nano composite material

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specific Embodiment approach 1

[0027] Specific implementation mode 1: In this implementation mode, LDHSiO 2 The synthesis of shell-core nanocomposites is achieved by the following steps:

[0028] 1. At room temperature, add 90mL of absolute ethanol, 10mL of deionized water and 2-4mL of ammonia water in sequence, and stir evenly with magnetic force; add 10mL of ethyl orthosilicate at one time under the condition of magnetic stirring, and continue to stir at room temperature for 2.0-2.5 h, after that, let it stand for 10-12 hours; then centrifuge for 3-5 minutes at a speed of 10000-12000r / min, collect the precipitate, and wash the precipitate repeatedly with deionized water and absolute ethanol until the pH of the washed liquid It is neutral, collect the washed precipitate and dry it in an oven at 50-65°C to obtain SiO 2 Nanoparticles;

[0029] Two, take 0.5g of SiO prepared in step one 2 Nanoparticles dispersed to 10mL for CO removal 2 Ultrasonic dispersion in deionized water for 5-10 minutes to obtain S...

specific Embodiment approach 2

[0032] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the amount of ammonia water in step one is 2mL. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0033] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the volume percentage of ammonia water in Step 1 is 20-30%. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a synthetic method of an LDH@SiO2 shell-nuclear nano composite material. The method comprises the following steps: I, magnetically stirring and uniformly mixing anhydrous ethanol, deionized water and ammonia water, then, adding ethyl orthosilicate, continuously stirring, standing, centrifuging, collecting precipitates, washing to neutral and drying; II, dissolving NaOH and NaOH3 in deionized water with CO2 removed and dispersing SiO2 nano particles therein to obtain liquid A; III, dissolving 15-20g of Mg(NO3)3.6H2O and 10-12g of Al(NO3)3.9H2O in deionized water without CO2 to obtain liquid B; and IV, dropwise adding the liquid B into the liquid A while stirring, stirring at constant temperature, washing the precipitates by using the deionized water without CO2, and drying in vacuum to obtain the material. The material provided by the invention is uniform in particle size distribution and easy to control, simple in preparation process, relatively low in production cost and easy for scaled production.

Description

technical field [0001] The present invention relates to LDHSiO 2 Shell-core nanocomposite material synthesis and method for preparing Newcastle disease virus La Sota strain F gene plasmid DNA nanoparticles. Background technique [0002] As a new type of gene delivery and controlled release system, novel nanocomposites have received more and more attention. Nanocarriers have many advantages, such as protecting DNA from nuclease degradation; good osmotic effect, can directly enter cells through biofilm barriers, release plasmid DNA, and express corresponding proteins; effectively uptake by antigen-presenting cells, and extract after processing. Presented to T cells to generate an immune response, etc. [0003] Layered double hydroxides (LDHs), a class of anionic clays with anion-exchangeable properties, have been widely used as precursors for intercalation reactions and are regarded as a new class of delivery vehicles for drug molecules. Studies have shown that as a new typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/12C01F7/02B82Y30/00A61K48/00A61K47/04A61K39/17A61P31/14C12N15/63
Inventor 赵凯王晓华
Owner HEILONGJIANG UNIV
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