Application of chicken-derived small molecule peptide in preparation of product for preventing and improving liver injury and secondary symptoms thereof and product

A small molecule peptide, liver injury technology, applied in the field of food and medicine and health, can solve the problems of liver complexity, aggravation of liver injury, difficult to cure, etc., to improve liver injury and related secondary symptoms, improve liver volume reduction, The effect of high safety

Active Publication Date: 2022-05-13
完美(广东)日用品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to incomplete statistics, there are currently more than 1,000 drugs with potential hepatotoxicity in clinical practice, and drug excipients, traditional Chinese medicines, and health products also have the possibility of inducing drug-induced liver injury, and recent studies have found that liver ...

Method used

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  • Application of chicken-derived small molecule peptide in preparation of product for preventing and improving liver injury and secondary symptoms thereof and product
  • Application of chicken-derived small molecule peptide in preparation of product for preventing and improving liver injury and secondary symptoms thereof and product
  • Application of chicken-derived small molecule peptide in preparation of product for preventing and improving liver injury and secondary symptoms thereof and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The extraction method of CPP comprises the following steps:

[0057] 1) Preparation of chicken powder: take fresh chicken and cut it into pieces and dry it, then use a pulverizer to pulverize it at 10000rpm / min, every pulverization is 2 minutes, pause for 1 minute, after accumulative pulverization for 5 minutes, dry powder is obtained, and then the powder is passed through 40 After the mesh sieve, the part under the sieve is the chicken powder.

[0058] 2) Extraction of small molecular peptides: Weigh 135 g of the chicken powder prepared above, then add an acetic acid solution with a concentration of 1 mole / liter (mol / L) to prepare a chicken acetic acid solution with a concentration of 200 mg / mL, and then add an equal volume of The ether solution was shaken immediately to fully extract, and after standing for 10 minutes, the ether layer solution was collected and dried with a rotary evaporator, and the obtained dry powder was the crude small molecule peptide powder.

...

Embodiment 2

[0064] Determination of in vitro antioxidant capacity of CPP: The in vitro antioxidant capacity of CPP was determined by the Oxygen Radical Absorbance Capacity (ORAC) test.

[0065] ORAC experiment process: 20 μl of aqueous solutions containing different concentrations of CPP were added to the 96-well plate to set up the experimental group, and the concentrations of CPP were 0.4mmol / L, 0.8mmol / L and 1.5mmol / L respectively, and a blank control group and a model group were set up at the same time. Add 40 μl of oxidative stress inducer AAPH to the experimental group and model group, add 20 μl phosphate buffer saline to all groups, and finally add 20 μl sodium luciferin to all groups and quickly place the 96-well plate in luciferase at a set temperature of 37°C Start the measurement in the standard instrument, and measure a point every 2 minutes for a total of 2 hours. The ORAC value is calculated by using the protection integral area corresponding to 1 μmol / L Trolox on the fluores...

Embodiment 3

[0068] Determination of the effect of CPP on the viability of liver cells:

[0069] In this example, the cell viability is detected by the classic MTT experiment. MTT is a yellow powder, the full name of which is thiazolium. The detection principle is that MTT can penetrate the cell membrane into the cell and be reduced to insoluble The blue-purple needle-like crystal formazan in water is deposited in the cells, but dead cells do not have this function; the crystals formed by living cells can be dissolved by dimethyl sulfoxide (DMSO), and the light absorption value detected by it can be indirectly Reflect the status of cell survival and growth, so that the toxic and side effects of drugs can be evaluated.

[0070] The specific experimental process is as follows:

[0071] 1) Select a human liver cancer cell line HepG2 in good condition, in the logarithmic growth phase, with a growth density of about 80% in the culture dish, spread it in a 96-well plate with 7,000 cells per wel...

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PUM

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Abstract

The invention discloses application of chicken-derived small molecule peptide in preparation of a product for preventing and improving liver injury and secondary symptoms thereof and the product, and relates to the field of food and medicine health. The small molecule peptide is a chicken-derived natural free active peptide, the small molecule peptide is specifically dipeptide Cyclo (L-Phe-L-Phe), the small molecule peptide shows strong antioxidant ability in cells, the oxidative stress state of the liver can be improved through the effects of increasing the activity of antioxidant enzyme GPx, reducing the level of oxidative stress product malonaldehyde MDA and the like, and the liver protection effect is achieved. The CPP has the advantages of low toxicity, wide medication window and high safety, and can be applied to functional foods, health foods, pharmaceuticals, compositions and other products for preventing or improving liver injury and related secondary symptoms thereof.

Description

technical field [0001] The invention relates to the fields of food, medicine and health, in particular to the application of a chicken-derived small molecule peptide in the preparation of products for preventing and improving liver damage and its secondary symptoms and the product. Background technique [0002] As the main metabolic organ of the human body, the liver participates in the processes of digestion, metabolism, excretion, detoxification and immunity in the body, and its normal function is crucial to human health. With the rapid development of social economy and the change of living environment, people's psychological pressure is increasing, and people often drink alcohol to relieve the pressure, and the number of patients with alcoholic liver injury is increasing year by year. In addition, environmental pollution and food and drug safety incidents are high, and liver dysfunction and organic damage are also increasing, especially drug-induced liver injury. [0003...

Claims

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Application Information

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IPC IPC(8): A61K38/05A61P1/16A21D2/26A23C19/09A23G1/44A23G3/44A23G4/14A23L2/66A23L11/45A23L21/10A23L33/18
CPCA61K38/05A61P1/16A23L33/18A23L2/66A21D2/268A23G1/44A23G3/44A23G4/14A23L21/10A23L11/45A23C19/0921A23V2002/00A23V2200/30A23V2250/55
Inventor 张琼谊李晓敏严建刚王宝东余宗盛卢海英栗原博古润金
Owner 完美(广东)日用品有限公司
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