Fucoidan amino acid compound, pharmaceutical application thereof, health food containing fucoidan amino acid compound, cosmetic and fucoidan amino acid medicine compound containing fucoidan amino acid compound
A technology of amino acid and fucoidan, which is applied in the direction of cosmetics, drug combinations, cosmetic preparations, etc., can solve the problems of cell internalization restrictions, achieve the effect of strengthening the degree of cell internalization, improving the degree of cell internalization, and modifying the appearance
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preparation example 1
[0053] Preparation Example 1: Small Molecule Fucoidose
[0054] The extraction method of this experiment is to put 100 g of dry algae into 5 liters of distilled water and boil them in boiling water at 100°C for 30 minutes, then centrifuge the hot water extract, and freeze the supernatant under reduced pressure. After drying (lyophilized), 95% ethanol was added and left standing at 4°C overnight to obtain a precipitate. The precipitated polysaccharides were further collected by centrifugation, and then lyophilized to obtain the primary extract of algae. Finally, the primary extract of algae is further hydrolyzed with enzymes to obtain low-molecular-weight fucoidose, and the low-molecular-weight fucoidose contains a hetero-oligosaccharide, and the average molecular weight of the hetero-oligosaccharide is 400 Daltons to 1500 Daltons pause. The fucoidose used in this experiment was low molecular weight fucoidan produced by Taiwan Chunghwa Marine Biotechnology Co., Ltd., and it w...
preparation example 2
[0055] Preparation Example 2: Fucoidose L-Amino Acid Complex
[0056] The small molecule fucoidose and L-arginine were mixed in a weight ratio of 1 to 1, 1 to 2.5 and 1 to 5, respectively, and after stirring at 300 rpm for about 12 hours, the molecular weight cutoff (MWCO) 1000 Dalton (Da) (ie, 1000 MWCO) cellulose dialysis membrane was purified in deionized water for 1 day, and then freeze-dried to obtain a fucoidose L-amino acid complex.
preparation example 3
[0057] Preparation Example 3: Fluorescent Complex
[0058] (1) Fucoidose Fluorescent Complex: Mix 1 mmol (mmol) of small molecule fucose, 3 mmol of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (1-ethylaminopropyl) -3-(3-dimethylaminopropyl)-carbodiimide (EDC) and 4 mmol of N-Hydroxysuccinimide (NHS) in 10 mL of dichloromethane (DCM) solvent and stirred for 12 hours , so that NHS and the carboxyl group (C(=O)OH)) of the small molecule fucose are bonded to form an ester group. After that, 8 mmol of ethylenediamine (EDA) and 200 μL of pyridine (pyridine) were added, and stirred for 24 hours to aminate the small molecule fucose, and then purified in deionized water with a 1000 MWCO dialysis bag for 2 days, and frozen. After drying, an aminated small molecule fucose is obtained. Mix 18 mmol of aminated small molecule fucose and 0.2 mmol of Cy3 NHS ester dye (Cyanine 3(Cy3) NHS ester dye, purchased from Hallandale Beach, USA) dissolved in 10 mL of DCM, and stir for 24 hours, and ...
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