Polymer-entrapped erythrocyte membrane composite material as well as preparation method and application thereof

A technology of red blood cell membrane and composite materials, which is applied in the field of red blood cell membrane composite materials and its preparation, can solve problems such as mutual aggregation, complicated process, and limitations, and achieve good deformation ability, simple preparation method, and uniform particle size.

Active Publication Date: 2022-05-31
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the process of extracting the cell membrane by this method is complicated. After the cell membrane is cracked and wrapped on the surface of the nanoparticles, it is easy to make the particles aggregate with each other, which limits the application of this method to a certain extent.

Method used

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  • Polymer-entrapped erythrocyte membrane composite material as well as preparation method and application thereof
  • Polymer-entrapped erythrocyte membrane composite material as well as preparation method and application thereof
  • Polymer-entrapped erythrocyte membrane composite material as well as preparation method and application thereof

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Experimental program
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Embodiment

[0070] This embodiment provides a polymer-loaded erythrocyte membrane composite material and a polymer-loaded erythrocyte membrane composite material with targeting properties. The preparation method is as follows:

[0071] (1) Take 5 ml of sterile defibrinated mouse blood and add 5 ml of 1×PBS solution. Place in a high-speed centrifuge at 4°C and centrifuge at 3,000 rpm for 5 minutes, then wash with 1×PBS solution for 3 times to obtain a pure erythrocyte suspension;

[0072] (2) Resuspend the erythrocytes obtained in step (1) in 0.25×PBS solution, lyse at 4°C for 20 min, and wash 3 times with 0.25×PBS solution to obtain erythrocyte membrane suspension;

[0073] (3) Add 20 mg of dopamine hydrochloride to the erythrocyte membrane suspension obtained in step (2), and stir at 4° C. for 4 hours to obtain the desired micro-nano particle precursor;

[0074] (4) Centrifuge, resuspend the centrifuged substrate in 10×PBS solution with 25% ammonia water, stir at 4°C for 6 hours to obta...

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Abstract

The invention relates to a polymer-entrapped red cell membrane composite material as well as a preparation method and application thereof. The polymer-entrapped red cell membrane composite material comprises red cell membrane vesicles and in-situ formed polymer particles entrapped in the red cell membrane vesicles. The invention develops a system capable of carrying out polymerization reaction in the erythrocyte membrane vesicles, the system takes the cavitated erythrocyte membrane vesicles as a micro-reactor, reaction monomers of the polymerization reaction are loaded into the erythrocyte membrane vesicles, and the polymerization reaction is initiated in the erythrocyte membrane vesicles under mild conditions, so that the erythrocyte membrane vesicles can be obtained. The complete structure of an erythrocyte membrane is reserved to the greatest extent, namely, the natural biocompatibility, large inner cavity, good deformability and immunogenicity deficiency of red blood cells are reserved, and the loading efficiency of functional components can be improved. Various functional groups can be modified on the surface, and the application scene is wide.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a polymer-loaded erythrocyte membrane composite material and a preparation method and application thereof. Background technique [0002] Red blood cells are the most numerous cells in the human body and play an important role in transporting oxygen and carbon dioxide in the human body. Because erythrocytes have natural biocompatibility, large lumen, good deformability, and lack of immunogenicity, erythrocytes are also commonly used as drug loading and transport vehicles for the application research of anti-tumor therapy. For example, it has been reported that the large specific surface area and charge of red blood cells are used to load drugs and transport them to the lesion for anti-tumor therapy. This method is called "hitchhiking". In addition, there are also studies that load small-molecule drugs in the extremely large cavity of red blood cells, and release the dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61K47/62A61K49/00A61P35/00
CPCA61K9/5068A61K41/0052A61K47/62A61P35/00A61K49/0019A61K49/0097A61K49/0056Y02A50/30
Inventor 杨洋白时唯李春梅李峻柏
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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