Method for inactivating biological soft tissue virus

Abstract

The invention provides a method for inactivating a biological soft tissue virus, which comprises the following steps of: 1, putting a biological soft tissue into an inactivation container; step 2, immersing the biological soft tissue in the inactivation container with 0.15-0.2% of peracetic acid and 2-8% of an ethanol solution at the temperature of 20-30 DEG C for 0.5-4 hours; 3, purified water is added for cleaning until the PH of the eluent in the inactivation container is neutral and the content of peracetic acid is lower than 1 mg / L, and an inactivated tissue X is obtained; 4, irradiating the inactivated tissue X under the irradiation of electron beams with the irradiation dose of 15-25KGy to obtain inactivated tissue Y; the method has the advantages that on one hand, viruses in tissues are effectively removed, and on the other hand, the complete structure of the tissues is reserved.

Description

technical field [0001] The invention relates to the field of virus inactivation, in particular to a method for inactivating biological soft tissue viruses. Background technique [0002] Skin and soft tissue injuries are prone to infection, which is caused by the reproduction and derivation of bacteria or viruses. The most common is the inflammatory disease caused by suppurative pathogenic bacteria invading the epidermis, dermis and subcutaneous tissue. In the field of biomedicine, for research biological The study of soft tissue viruses needs to be inactivated, so the method of biological soft tissue inactivation is also more important. The existing inactivation methods are various, but the current existing methods have low virus inactivation efficiency, and the degree of inactivation can reach Less than the specified requirements, the inactivation steps are complicated and time-consuming. [0003] The existing technologies (patent numbers: CN104524606A, CN102210854B, CN102...

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Problems solved by technology

[0002] Skin and soft tissue injuries are prone to infection, which is caused by the reproduction and derivation of bacteria or viruses. The most common is the inflammatory disease caused by suppurative pathogenic bacteria invading the epidermis, dermis and subcutaneous tissue. In the field of biomedicine, for research biological The study of soft tissue viruses needs to be inactivated, so the method of biological soft tissue inactivation is also more important. The existing inactivation methods are various, but the current existing methods have low virus inactivation efficiency, and the degree of inactivation can reach Less than the specified requirements, the inactivation steps are complicated and time-consuming

[0003] The existing technologies (patent numbers: CN104524606A, CN102210854B, CN102286095B, ​​USP5116950, and USP5639730) all use heat-inactivated hair, and the temperature ranges from 60°C to 121°C, but for biological tissues, denaturation tends to occur when the temperature exceeds 40°C , irreversible changes can occur at 60°C; wherein (patent number: CN103785065A) discloses the use of peracetic acid-ethanol solution to treat cartilage tissue, but has not verified its virus inactivation effect on soft tissues

In addition, only one-step inactivation process is introduced, which does not meet the requirements of the regulations issued by NMPA. Among them (patent number: CN103648508A) discloses the use of SD inactivation method, mainly for blood products, but does not verify its virus inactivation effect on soft tissues ; Wherein (patent No. is: CN103191466A) discloses using solutions such as peroxyacetic acid, sodium chloride, surfactant and caustic soda to carry out deactivation, and step is loaded down with trivial details, and the time is long, and caustic soda solution is harmful to human body

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