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Probiotic composition and application thereof

A composition and probiotic technology, applied in the field of microorganisms, to achieve the effects of improving symptoms, good inhibitory effect, and environmental protection of feed

Pending Publication Date: 2022-06-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no relevant reports on the use of probiotic compositions for the prevention and treatment of European larval rot and bee food additives

Method used

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  • Probiotic composition and application thereof
  • Probiotic composition and application thereof
  • Probiotic composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1: Preparation of freeze-dried inoculum

(1) Lactobacillus W8173 was activated with MRS medium, Bifidobacterium W8118 was activated with TPY medium, and the inoculum was 3%. The activated strains were transferred to MRS liquid medium and 4% inoculum respectively In TPY liquid medium, cultured at 37 °C for 25 h, centrifuged (5000 g, 15 min) to harvest the cells for later use; the above cells were added in a protective agent (1% trehalose, glutamine, 1% trehalose, glutamine) in a volume ratio of 3:3:2. Sodium 0.5%, skim milk 1%, ascorbic acid 0.25%, dextran 0.25%, g / 100mL distilled water), mix well and set aside. Before freeze-drying, the samples were pre-frozen in a -80°C ultra-low temperature freezer for 3 hours, followed by three-stage freeze-drying at 512 Pa for 6 hours, then at 256 Pa for 5 hours, and then at 103 Lyophilized under Pa until a powder product was obtained. The freeze-dried bacterial preparations were stored at 4 °C and 25 °C, respectively, and the via...

Embodiment 2

[0014] Example 2: In vitro antibacterial experiment of mixed bacterial powder

(1) Configuration of the aseptic supernatant of the mixed bacterial powder of the test product: the mixed bacterial powder in the above experiment was lyophilized and inoculated into the BHI liquid medium for 5 days, and the culture solution was passed through a low-temperature high-speed centrifuge 8000g Centrifuge for 10 min, take the supernatant, and finally filter the supernatant through a 0.22 μm pore filter to obtain a sterile supernatant of the probiotic composition;

(2) Culture and inhibition zone experiment of pathogenic bacteria Bee coccus apis

Add 5 mL of 1×PBS buffer to a 10 mL centrifuge tube, scrape all the honey bee coccus in the best growth state on the plate into the centrifuge tube with an inoculation loop, and shake it evenly with a vortex shaker to prepare a bacterial suspension. Take 50 μL of the bacterial suspension and place it on the KSBHI plate and spread it evenly; prepa...

Embodiment 3

[0016] Example 3: In vitro bacteriostatic test of probiotic composition against Staphylococcus aureus and Faecalis

(1) The preparation of sterile supernatant is the same as step (1) in Example 2;

(2) Culture and inhibition zone experiment of Staphylococcus aureus and Faecalicoccus

Add 5 mL of 1×PBS buffer to a 10 mL centrifuge tube, scrape all the Staphylococcus aureus and Faecalicoccus in the optimal growth state on the plate into 2 centrifuge tubes with an inoculating loop, and shake with a vortex shaker Evenly, prepare a bacterial suspension, take 50 μL of the bacterial suspension and place it on the BHI plate and spread it evenly; prepare 10 mm holes on the coated agar medium; take 100 μL of sterile supernatant and place them on the prepared Good Staphylococcus aureus and faecalis in the wells; place the plate in 5% CO 2 Incubate for 3-4 days at 35°C in an incubator; observe whether there is the formation of an inhibition zone around the well;

Depend on figure 2 a...

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Abstract

The invention discloses a probiotic composition and an application of the probiotic composition. The probiotic composition is prepared from lactobacillus (Lactobacillus sp.) W8173 and bifidobacterium (Bifidobacterium sp.) W8118, and the probiotic composition is prepared from the following raw materials of lactobacillus (Lactobacillus sp.) W8173, bifidobacterium (Bifidobacterium sp.) W8118, lactobacillus (Lactobacillus sp.) W8173 and bifidobacterium (Bifidobacterium sp. The probiotic composition can improve symptoms of European foulbrood of bees, can inhibit growth of honeycomb bee coccus, staphylococcus aureus and faecalis coccus in an in-vitro bacteriostasis experiment, is environment-friendly and pollution-free, does not influence the quality of bee products, can be used as a microbial inoculum, a feed or a bee food additive, and can be applied to the field of breeding of bees. The method is suitable for industrial production and market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a probiotic composition and application thereof. Background technique [0002] Bees are important economic insects and play an important role in the process of constructing and maintaining natural ecosystems and ecological agriculture, and supplying human advanced nutritional and health-care functional products. Of the food crops used by humans, 76% rely on bees for pollination. The economic benefits of bee pollination are 10 to 15 times higher than the value of honey production. On May 6, 2019, the authoritative report released by the United Nations Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) shows that due to the disappearance of pollinators, global crop production faces an annual loss of about 235-577 billion US dollars. However, with the aggravation of the imbalance of the ecological environment, the threat of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/90A01N63/20A01P1/00C12R1/225C12R1/01
CPCC12N1/20A23K10/18A23K50/90A01N63/20A23V2400/11A23V2400/51Y02A50/30
Inventor 郑浩段惠娟郎浩宇
Owner CHINA AGRI UNIV
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