Probiotic composition and application thereof
A composition and probiotic technology, applied in the field of microorganisms, to achieve the effects of improving symptoms, good inhibitory effect, and environmental protection of feed
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
Example 1: Preparation of freeze-dried inoculum
(1) Lactobacillus W8173 was activated with MRS medium, Bifidobacterium W8118 was activated with TPY medium, and the inoculum was 3%. The activated strains were transferred to MRS liquid medium and 4% inoculum respectively In TPY liquid medium, cultured at 37 °C for 25 h, centrifuged (5000 g, 15 min) to harvest the cells for later use; the above cells were added in a protective agent (1% trehalose, glutamine, 1% trehalose, glutamine) in a volume ratio of 3:3:2. Sodium 0.5%, skim milk 1%, ascorbic acid 0.25%, dextran 0.25%, g / 100mL distilled water), mix well and set aside. Before freeze-drying, the samples were pre-frozen in a -80°C ultra-low temperature freezer for 3 hours, followed by three-stage freeze-drying at 512 Pa for 6 hours, then at 256 Pa for 5 hours, and then at 103 Lyophilized under Pa until a powder product was obtained. The freeze-dried bacterial preparations were stored at 4 °C and 25 °C, respectively, and the via...
Embodiment 2
[0014] Example 2: In vitro antibacterial experiment of mixed bacterial powder
(1) Configuration of the aseptic supernatant of the mixed bacterial powder of the test product: the mixed bacterial powder in the above experiment was lyophilized and inoculated into the BHI liquid medium for 5 days, and the culture solution was passed through a low-temperature high-speed centrifuge 8000g Centrifuge for 10 min, take the supernatant, and finally filter the supernatant through a 0.22 μm pore filter to obtain a sterile supernatant of the probiotic composition;
(2) Culture and inhibition zone experiment of pathogenic bacteria Bee coccus apis
Add 5 mL of 1×PBS buffer to a 10 mL centrifuge tube, scrape all the honey bee coccus in the best growth state on the plate into the centrifuge tube with an inoculation loop, and shake it evenly with a vortex shaker to prepare a bacterial suspension. Take 50 μL of the bacterial suspension and place it on the KSBHI plate and spread it evenly; prepa...
Embodiment 3
[0016] Example 3: In vitro bacteriostatic test of probiotic composition against Staphylococcus aureus and Faecalis
(1) The preparation of sterile supernatant is the same as step (1) in Example 2;
(2) Culture and inhibition zone experiment of Staphylococcus aureus and Faecalicoccus
Add 5 mL of 1×PBS buffer to a 10 mL centrifuge tube, scrape all the Staphylococcus aureus and Faecalicoccus in the optimal growth state on the plate into 2 centrifuge tubes with an inoculating loop, and shake with a vortex shaker Evenly, prepare a bacterial suspension, take 50 μL of the bacterial suspension and place it on the BHI plate and spread it evenly; prepare 10 mm holes on the coated agar medium; take 100 μL of sterile supernatant and place them on the prepared Good Staphylococcus aureus and faecalis in the wells; place the plate in 5% CO 2 Incubate for 3-4 days at 35°C in an incubator; observe whether there is the formation of an inhibition zone around the well;
Depend on figure 2 a...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap